生长抑素受体亚型在人肝星状细胞的表达及其临床意义

首都医科大学学报 ›› 2006, Vol. 27 ›› Issue (4): 441-444.

生长抑素受体亚型在人肝星状细胞的表达及其临床意义

张娟¹, 丁惠国², 尤红³, 唐淑珍³, 尚宏伟⁴, 王兴翠⁴, 贾继东³

1. 首都医科大学附属北京佑安医院肝病消化科;2. 首都医科大学附属北京友谊医院肝病中心;3. 首都医科大学基础医学院

收稿日期:2006-06-16 修回日期:1900-01-01 出版日期:2006-08-24 发布日期:2006-08-24
通讯作者: 张澍田

Expressions of Subtypes of Somatostatin Receptors in Activated Human Hepatic Stellate Cells and Its Clinical Significance

Zhang Juan¹, Ding Huiguo², You Hong³, Tang Shuzhen³, Shang Hongwei⁴, Wang Xingcui⁴, Jia Jidong³

1. Department of Hepatology and Gastroenterology, Beijing You'an Hospital, Capital University of Medical Sciences;2. Liver Research Center, Beijing Friendship Hospital;3. School of Basic Medical Sciences, Capital University of Medical Sciences

Received:2006-06-16 Revised:1900-01-01 Online:2006-08-24 Published:2006-08-24

摘要/Abstract

摘要： 目的研究人肝星状细胞系(LX-2细胞)生长抑素受体(somatostatin receptor, SSTR)亚型的表达情况,探讨其临床意义.方法选择对数生长期的LX-2细胞,将细胞以8×10⁴个/孔接种于预置入盖玻片的6孔板中,分为LX-2组、中药复方861组、奥曲肽(octreotide)处理组及SSTR亚型阳性细胞(PANC-1)对照组,每组重复2孔.应用免疫细胞化学染色方法,观察LX-2细胞SSTR 2、SSTR5的表达;用RT-PCR方法观察SSTR亚型1～5的表达.结果免疫细胞化学染色结果发现,LX-2细胞SSTR亚型2、5表达阳性,阳性物质呈棕红色,主要位于细胞膜及细胞质,复方861处理组SSTR2、5表达增强,细胞着色较深;RT-PCR结果发现,LX-2细胞SSTR亚型1～5的表达均阳性,阳性表达的高低依次为SSTR1、SSTR4＞SSTR2、SSTR5＞SSTR3(P＜0.05).结论 LX-2表达5种SSTR亚型,肝星状细胞可能是SST及其类似物降低门静脉压力的作用靶点.奥曲肽可能通过SSTR2、 SSTR5亚型调节肝星状细胞的舒张与收缩功能,从而影响肝内阻力及门静脉压力.

关键词: 肝星状细胞, 生长抑素受体亚型, 复方861, 奥曲肽

Abstract: Objective To study the expressions of somatostatin receptor subtypes in human activated hepatic stellate cells(LX-2）.Its clinical significances was also surveyed.Methods To detect the expressions of SSTR2/5 of LX-2 which is in the logarithmic growth cycle using immunocytochemistry staining method: LX-2 were routinely cultured on small glass coverslip in six well cluster dishes at a density of 8×10⁴ cells/well.The cells were divided into 5 subgroups: compound 861 0.5 g/L,compound 861 1.0 g/L,octreotide 1 mg/L and control group,PNAC-1 was a positive control cell line.Meantime,expressions of SSTR1-5 mRNA were observed using RT-PCR method.Results The expressions of SSTR 2,SSTR5 in LX-2 were positive after immunocyto-chemistry staining.The mainly staining site is cellular membrane and cellular plasm.The strong expressions in compound 861-treated group were also observed.The five kinds of subtypes of SSTR in LX-2 were proven to be positive via the detection of RT-PCR.To compared the level of the subtypes,SSTR1,4>SSTR2,5>SSTR3 was observed.Conclusion LX-2 expresses five kinds of somatostatin receptors subtype.It is suggested that the activated hepatic stellate cells is target of SST and its analogs.

Key words: hepatic stellate cell, somatostatin receptor subtype, compound 861, octreotide

中图分类号:

R657.3⁺4

张娟;丁惠国;尤红;唐淑珍;尚宏伟;王兴翠;贾继东. 生长抑素受体亚型在人肝星状细胞的表达及其临床意义[J]. 首都医科大学学报, 2006, 27(4): 441-444.

Zhang Juan;Ding Huiguo;You Hong;Tang Shuzhen;Shang Hongwei;Wang Xingcui;Jia Jidong. Expressions of Subtypes of Somatostatin Receptors in Activated Human Hepatic Stellate Cells and Its Clinical Significance[J]. Journal of Capital Medical University, 2006, 27(4): 441-444.

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