急性脑损伤致心肌损害中心肌G蛋白表达的改变

doi:10.3969/j.issn.1006-7795.2015.01.021

首都医科大学学报 ›› 2015, Vol. 36 ›› Issue (1): 111-115.doi: 10.3969/j.issn.1006-7795.2015.01.021

急性脑损伤致心肌损害中心肌G蛋白表达的改变

郭彩霞¹, 曾翔俊², 杜凤和¹, 陈步星¹

1. 首都医科大学附属北京天坛医院心内科, 北京 100050;
2. 首都医科大学基础医学院病理生理学教研室, 北京 100069

收稿日期:2014-10-08 出版日期:2015-02-21 发布日期:2015-01-31
通讯作者: 郭彩霞 E-mail:cxgbb@163.com
基金资助:
国家自然科学基金(81370313,30740054);北京市科技新星计划(2010B050);北京市卫生系统高层次卫生技术人才培养计划(2013-3-046)资助项目.

Change of G-protein expression in the myocardial damage induced by acute brain injury

Guo Caixia¹, Zeng Xiangjun², Du Fenghe¹, Chen Buxing¹

1. Department of Cardiology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China;
2. Department of Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2014-10-08 Online:2015-02-21 Published:2015-01-31
Supported by:
This study was supported by National Natural Science Foundation of China(81370313,30740054); Beijing NOVA Program(2010B050); Beijing Health System High Level Health Technical Personnel Training Plan(2013-3-046)

摘要/Abstract

摘要： 目的建立动物急性脑损伤(acute brain injury,ABI)模型,观察心肌G_s蛋白α亚基(α subunite of G_s, G_sα)和 G_q蛋白α亚基(α subunite of G_q,G_qα)的变化,探讨G_s和G_q在急性脑损伤致心肌损伤中的作用及其β₁肾上腺素受体阻断剂(adrenalin β₁ receptor blocker, β₁-ARB)和5-羟色胺受体2A阻断剂(5-hydroxytryptamine 2A receptor blocker, 5-HT_2ARB)对G蛋白的影响.方法复制Wistar大鼠ABI模型(n=8).采用数字表法随机分为正常组(NORM)、假手术对照组(SHAM)、急性脑损伤模型组(ABI)、β1-ARB组(BETA)和5-HT_2ARB组(KETA组).利用RT-PCR方法测定G_sα和G_qα mRNA表达;通过Western blotting方法测定G_sα和G_qα蛋白表达.结果与正常对照组(NORM:100%)相比,SHAM组、ABI组和BETA组G_sα mRNA表达分别为(92.6±24)%、(39.7±30)%和(80.1±12)%.ABI组G_sα mRNA表达比SHAM组显著降低(P<0.05),预先应用β1-ARB的BETA组G_sα mRNA表达比ABI组增高(P<0.05),而与SHAM组比差异无统计学意义(P>0.05);与正常对照组(NORM:100%)相比,SHAM组、ABI组和BETA组G_qα蛋白表达分别为(105±30)%、(110±14)% 和(107±23)%,各组之间G_qα蛋白表达差异无统计学意义(P>0.05).结论 ABI组G_sα mRNA和G_sα蛋白表达水平于脑损伤后显著降低.提示急性脑损伤致心肌损伤的发生可能与抑制G_s蛋白介导的信号转导通路有关,G_s蛋白可能以G_sα蛋白表达量改变的方式参与了急性脑损伤致心肌损伤的发生过程.

关键词: 急性脑损伤, 心肌损害, 5-HT_2ARB, G_s蛋白α亚基, G_q蛋白α亚基

Abstract: Objective To test the effect of G_s-protein and G_q-protein on cardiac damage induced by acute brain injury, and the contribution of β1-ARB and 5-HT_2ARB to G-protein. Methods The Wistar rat model of acute brain injury was built. The mRNA level of G_sα and G_qα were evaluated with real time PCR, and the protein level of G_sα and G_qα were detected by Western blotting assay.Results Compared with NORM group, the mRNA level of G_sα was (92.6±24)% in SHAM group, (39.7±30)% in ABI group and (80.1±12)% in BETA group, respectively. The mRNA level of G_sα was significantly decreased in ABI group compared to SHAM group, but significantly increased in β1-ARB treated BETA group, and there is no difference of G_sα mRNA level between β1-ARB treated BETA and SHAM groups. Compared with NORM group, the protein level of G_qα was (105±30)% in SHAM group, (110±14)% in ABI group and (107±23)% in BETA group. There was no statistical difference among three groups. Conclusion The mRNA and protein of G_sα were significantly decreased after acute brain injury, which indicates the occurrence of myocardial damage induced by acute brain injury may be related to the inhibition in signal transduction pathway mediated G_s-protein. G_s-protein may be in the form of expression change involved in the occurrence of myocardial damage induced by acute brain injury.

Key words: brain injury, myocardial damage, 5-HT_2ARB, G_sα-protein, G_qα-protein

中图分类号:

郭彩霞, 曾翔俊, 杜凤和, 陈步星. 急性脑损伤致心肌损害中心肌G蛋白表达的改变[J]. 首都医科大学学报, 2015, 36(1): 111-115.

Guo Caixia, Zeng Xiangjun, Du Fenghe, Chen Buxing. Change of G-protein expression in the myocardial damage induced by acute brain injury[J]. Journal of Capital Medical University, 2015, 36(1): 111-115.

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急性脑损伤致心肌损害中心肌G蛋白表达的改变

Change of G-protein expression in the myocardial damage induced by acute brain injury

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