关键词: 急性脑梗死, 血小板膜糖蛋白, CD62p, 流式细胞术, ROC曲线

Abstract: Objective To explore the relationship between the expression rate of platelet membrane glycoprotein CD62p and acute cerebral infarction, draw ROC curve, determine the cutoff value, and clarify its clinical significance. Methods Flow cytometry that adopts FITC and PE double calibration was used to detect CD62p positive rates in the plasma of acute cerebral infarction group patients, then comparative analyses were performed between the high-risk group and the control group. The relationship between the CD62p and cerebral infarction was analyzed by drawing the ROC curve and it was confirmed that the CD62p positive rate was applicable to determine the best cutoff value of acute cerebral infarction. Results Acute cerebral infarction group had a significantly higher positive CD62p expression rate than the high risk group and the control group, which are all statistically significant (P<0.01). Dynamic observation of the CD62p positive expression rate of acute cerebral infarction group showed that plasma CD62p expression rate was the highest in 24 h, a trend of gradual decline on 7 d and 14 d, but remained higher than that of the high risk group and the control group on 14 d. The results are statistically significant (P<0.01). ROC curve drown by using CD62p positive expression rate showed that 5.20% was the cutoff point. The sensitivity was 92%, specificity 95%, positive predictive value 98%, negative predictive value of 73% and coincidence rate (accuracy) 92%. Conclusion In the patients of both the acute cerebral infraction group and high risk group, the platelet was kept high level, but the platelet activation in acute cerebral infarction group was higher, and in turn it can be speculated that the higher the positive expression rate of CD62p, the serious the patient,s condition. When the detected CD62p is higher than 5.2% after analyzing the ROC curve which is drawn based on CD62p positive expression, it indicates very high possibility of cerebral infraction. Therefore, measuring CD62p could predict the occurrence of acute cerebral infarction.

Key words: acute cerebral infarction, platelet membrane glycoprotein, CD62p, flow cytometry, ROC curve