内皮素-1及其受体拮抗剂对HSC-T6细胞Ⅰ型胶原mRNA表达的作用

首都医科大学学报 ›› 2007, Vol. 28 ›› Issue (1): 68-70.

内皮素-1及其受体拮抗剂对HSC-T6细胞Ⅰ型胶原mRNA表达的作用

张军¹, 张忠涛¹, 王宇¹, 王萍², 李建设¹, 周延忠¹

1. 首都医科大学附属北京友谊医院普通外科;2. 首都医科大学附属北京友谊医院病理生理学实验室

收稿日期:2006-05-22 修回日期:1900-01-01 出版日期:2007-02-24 发布日期:2007-02-24

Effect of Endothelin-1 and Its Receptor Antagonists on the Expression of Collagen-1 mRNA in HSC-T6 Cells

Zhang Jun¹, Zhang Zhongtao¹, Wang Yu¹, Wang Ping², Li Jianshe¹, Zhou Yanzhong¹

1. Department of General Surgery, Beijing Friendship Hospital, Capital Medical University;2. Department of Pathophysiology, Beijing Friendship Hospital, Capital Medical University

Received:2006-05-22 Revised:1900-01-01 Online:2007-02-24 Published:2007-02-24

摘要/Abstract

摘要： 目的检测与探讨内皮素-1及其受体拮抗剂对肝星状细胞(HSC-T6)细胞Ⅰ型胶原mRNA表达的作用和影响机制。方法采用反转录聚合酶链反应法(reverse transcription-polymerase chain reaction,RT-PCR),进行半定量分析。结果与空白对照组相比,ET-1组和ET-1+BQ-123组Ⅰ型胶原mRNA的表达显著升高(0.706±0.027、0.724±0.059比0.400±0.047,P<0.05);与ET-1组相比,ET-1+BQ-788组和ET-1+BQ-123+BQ-788组Ⅰ型胶原mRNA的表达显著降低(0.427±0.131、0.382±0.022比0.706±0.027,P<0.05)。结论 ET-1使HSC-T6细胞Ⅰ型胶原mRNA的表达明显升高,选择性ETB受体拮抗剂BQ-788能抑制ET-1引起的Ⅰ型胶原mRNA的高表达,而选择性ETA受体拮抗剂BQ-123则对其无明显作用。

关键词: 内皮素-1, 肝星状细胞, Ⅰ型胶原, 反转录多聚酶链反应

Abstract: Objective To study the effects of endothelin-1 and its receptor antagonists on the expression of collagen-1 mRNA in HSC-T6 cells. Methods RT-PCR analysis was used in the research. Results The expression of collagen-1 mRNA in ET-1 and ET-1+BQ-123 group was significantly higher than that of control group (0.706±0.027 and 0.724±0.059 vs 0.400±0.047, P<0.05). The expression in ET-1+BQ-788 group and ET-1+BQ-123+BQ-788 group was significantly lower than ET-1 group (0.427±0.131 and 0.382±0.022 vs 0.706±0.027, P<0.05). Conclusion ET-1 can induce high expression of collagen-1 mRNA in HSC. BQ-788, and the selective ETB receptor antagonist, can inhibit high expression of collagen-1 mRNA in HSC-T6 induced by ET-1.

Key words: endothelin-1, hepatic stellate cell, collagen type 1, RT-PCR

中图分类号:

R575.2

张军;张忠涛;王宇;王萍;李建设;周延忠. 内皮素-1及其受体拮抗剂对HSC-T6细胞Ⅰ型胶原mRNA表达的作用[J]. 首都医科大学学报, 2007, 28(1): 68-70.

Zhang Jun;Zhang Zhongtao;Wang Yu;Wang Ping;Li Jianshe;Zhou Yanzhong. Effect of Endothelin-1 and Its Receptor Antagonists on the Expression of Collagen-1 mRNA in HSC-T6 Cells[J]. Journal of Capital Medical University, 2007, 28(1): 68-70.

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