多色流式细胞术对60例成人急性髓系白血病免疫分型的研究

首都医科大学学报 ›› 2005, Vol. 26 ›› Issue (4): 491-494.

多色流式细胞术对60例成人急性髓系白血病免疫分型的研究

孙雪静, 万岁桂, 徐娟, 苏力, 田丁

首都医科大学宣武医院血液科

收稿日期:2004-06-17 修回日期:1900-01-01 出版日期:2005-08-24 发布日期:2005-08-24

Research on Immunophenotyping with Muti-color Flow Cytometry in Sixty Adults with Acute Myeloid Leukemia

Sun Xuejing, Wan Suigui, Xu Juan, Su Li, Tian Ding

Department of Hematology, Xuanwu Hospital, Capital University of Medical Sciences

Received:2004-06-17 Revised:1900-01-01 Online:2005-08-24 Published:2005-08-24

摘要/Abstract

摘要：

目的探讨成人急性髓系白血病(AML)多色流式细胞术免疫分型的特点。方法采用CD₄₅/SSC双参数散点图设门方法进行多色流式细胞术免疫表型分析。结果髓系抗原CD₁₃和CD₃₃在AML各亚型中均有很高的表达,阳性率分别为98.3%和85.0%.CD₁₄和CD₆₄在AML-M4及AML-Ms患者中的表达率较高.CD₁₄和CD₆₄在AML-M4的表达率分别为60.0%和100.0%,在AML-Ms的表达率分别为80.0%和100.0%,但CD₁₄的特异性要高于CD₆₄.AML患者CD₃₄、CD₁₁₇、HLA-DR的表达率分别为59.3%、84.3%和71.7%;AML-M₃患者CD₁₁₇表达率为76.9%,CD₃₄及HLA-DR呈低表达.AML患者淋系抗原CD₇和CD₁₉阳性率分别为20.0%和3.3%。结论采用CD₄₅/SSC双参数散点图设门方法,使成人AML免疫分型结果更可靠.CD₁₃、CD₃₃、CD₁₄可作为AML免疫分型的髓系单抗.CD₃₄及HLA-DR低表达为AML-M₃的特征.成人AML淋系抗原表达以CD₇较常见.

关键词: 白血病, 髓系, 急性, 免疫表型, 流式细胞术

Abstract:

Objective To evaluate the charateristics of immunophenotyping in adults with acute myeloid leukemia（AML） using multi-color flow cytometry.Methods Immunophenotyping was performed with multi-color flow cytometry using CD₄₅ /SSC gating.Results The myeloid antigen of CD₁₃ and CD₃₃ was highly expressed in all patients with AML. The positive rate was 98.3 % and 85.0% respectively. The expression of CD₁₄ and CD₆₄ in patients with AML-M₄ and AML-M₅ were also high. The positive rate was 60.0%, 100.0% in AML-M₄ and 80.0%, 100.0% in AML-M₅ respectively. The expression of CD₃₄ 、CD₁₁₇ and HLA-DR in patients with AML was 59.3%, 84.3% and 71.7% respectively. CD₁₁₇ was expressed in 76.9% of patients with AML-M₃. Expression of CD₃₄ and HLA-DR was low in patients with AML-M₃. CD₇ and CD₁₉ expression in patients with AML was 20.0% and 3.3% respectively.Conclusion The immunophenotyping results of adults with AML were more reliable with gating of CD₄₅ /SSC. Monoclonal antibodies of CD₁₃, CD₃₃ and CD₁₄ can be used to detect the immunophenotyping in AML. The expression of CD₃₄ and HLA-DR was low in patients with AML-M₃. The expression of CD₇ lymphoid-associated antigens is relatively common in adults with AML.

Key words: leukemia, myeloid, acute, immunophenotyping, flow cytometry

中图分类号:

R733.71

孙雪静;万岁桂;徐娟;苏力;田丁. 多色流式细胞术对60例成人急性髓系白血病免疫分型的研究[J]. 首都医科大学学报, 2005, 26(4): 491-494.

Sun Xuejing;Wan Suigui;Xu Juan;Su Li;Tian Ding. Research on Immunophenotyping with Muti-color Flow Cytometry in Sixty Adults with Acute Myeloid Leukemia[J]. Journal of Capital Medical University, 2005, 26(4): 491-494.

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