磷酸鞘胺醇受体在四氯化碳引起的肝纤维化小鼠肝脏的表达

首都医科大学学报 ›› 2010, Vol. 31 ›› Issue (5): 610-613.

磷酸鞘胺醇受体在四氯化碳引起的肝纤维化小鼠肝脏的表达

杨琳^1,2, 赵东旭^1*, 李丽英^2*

1. 北京理工大学生命学院生物工程系;2. 首都医科大学细胞生物学系, 肝脏保护与再生调节北京市重点实验室

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-10-21 发布日期:2010-10-21
通讯作者: 赵东旭，李丽英

Expression of Sphingosine 1 phosphate Receptors in Fibrotic Liver Induced by Carbon Tetrachloride

YANG Lin^1,2, ZHAO Dong-xu^1*, LI Li-ying^2*

1. Department of Bioengineering School of Life, Beijing Institute of Technology; 2. Department of Cell Biology, Municipal Laboratory for Liver Protection and Regulation of Regeneration, Capital Medical University

Received:1900-01-01 Revised:1900-01-01 Online:2010-10-21 Published:2010-10-21
Contact: ZHAO Dong-xu，LI Li-ying

摘要/Abstract

摘要：

目的研究四氯化碳（CCl₄）引起的肝纤维化小鼠肝组织中的磷酸鞘胺醇（sphingosine 1-phosphate，S1P）受体1、2、3（S1P_1-3）mRNA和蛋白含量的变化。方法制备小鼠CCl4肝纤维化模型，采用实时荧光定量聚合酶链反应方法测定小鼠肝组织S1P_1-3 mRNA表达情况；采用免疫印记（Western blotting）方法测定小鼠肝组织S1P_1-3蛋白表达情况。结果 CCl₄诱导小鼠肝纤维化2周或4周后，与对照组相比，小鼠肝组织的S1P₁ mRNA的表达差异无统计学意义，而S1P₂、S1P₃ mRNA的表达显著上调(P<0.05）；与之相对应，S1P₁的蛋白表达差异无统计学意义，而S1P₂、S1P₃的蛋白表达明显增加（P<0.05）。结论在CCl₄引起的小鼠肝纤维化形成过程中，S1P₂、S1P₃在mRNA和蛋白水平均显著上调，表明S1P相关受体在肝纤维化发生过程中起重要作用。

关键词: 肝脏纤维化, 磷酸鞘胺醇, 磷酸鞘胺醇受体

Abstract:

Objective To determine the mRNA and protein expression of sphingosine 1-phosphate receptors 1, 2 and 3(S1P_1-3) in the normal and fibrotic liver. Methods ICR mice were randomized into two experimental groups, control [olive oil(OO) treated] and model(CCl₄ treated) groups. Mice were sacrificed at two or four weeks of treatment. Liver tissue was examined by real-time RT-PCR and Western blotting to analyze the expression of S1P_1-3. Results Two or four weeks treatment by CCl₄, S1P₁ mRNA expression was not significantly different; S1P₂, S1P₃ mRNA expression was up-regulated(P<0.05) in liver tissue. Correspondingly, S1P₁ protein level was not significantly different; S1P₂, S1P₃ protein level was up-regulated(P<0.05). Conclusion During the process of liver fibrosis, sphingosine 1-phosphate receptor1-3 mRNA expression and protein level were various, the relative mRNA and protein level expression of S1P₂, S1P₃ have shown increases. Our results indicate that S1P receptors have an extremely important role in liver fibrogenesis.

Key words: liver fibrosis, sphingosine 1phosphate, sphingosine 1phosphate receptor

中图分类号:

杨琳;赵东旭;李丽英. 磷酸鞘胺醇受体在四氯化碳引起的肝纤维化小鼠肝脏的表达[J]. 首都医科大学学报, 2010, 31(5): 610-613.

YANG Lin;ZHAO Dong-xu;LI Li-ying. Expression of Sphingosine 1 phosphate Receptors in Fibrotic Liver Induced by Carbon Tetrachloride[J]. Journal of Capital Medical University, 2010, 31(5): 610-613.

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