高频电刺激吗啡精神依赖大鼠伏隔核后相关脑区细胞外氨基酸类神经递质的变化

首都医科大学学报 ›› 2010, Vol. 31 ›› Issue (5): 600-603.

高频电刺激吗啡精神依赖大鼠伏隔核后相关脑区细胞外氨基酸类神经递质的变化

胡文瀚¹, 张颖¹, 孟凡刚¹, 张斌², 张凯², 张建国^2*

1. 首都医科大学北京市神经外科研究所功能神经外科研究室;2. 首都医科大学附属北京天坛医院神经外科

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-10-21 发布日期:2010-10-21
通讯作者: 张建国

Changes of Extracellular Amino Acids Levels in Related Brain Regions after High Frequency Electrical Stimulation of Nucleus Accumbens in Morphine Psychic Dependent Rats

HU Wen-han¹, ZHANG Ying¹, MENG Fan-gang¹, ZHANG Bin², ZHANG Kai², ZHANG Jian-guo^2*

1. Stereotactic and Functional Neurosurgery Laboratory, Beijing Neurosurgical Institute, Capital Medical University;2. Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University

Received:1900-01-01 Revised:1900-01-01 Online:2010-10-21 Published:2010-10-21
Contact: ZHANG Jian-guo

摘要/Abstract

摘要：

目的通过研究高频电刺激吗啡精神依赖大鼠伏隔核（nucleus accumbens，NAc）后，NAc、苍白球腹侧核（ventral pallidum，VP）以及中脑腹侧被盖区（ventral tegmental area，VTA）中细胞外谷氨酸（glutamate，Glu）和γ氨基丁酸（gamma-aminobutyric acid，GABA）含量的变化，阐述高频电刺激治疗药物精神依赖的机制。方法 Wistar大鼠皮下注射盐酸吗啡10 d（3次/ d），对照组大鼠在同等时间给予同体积的0.9%氯化钠注射液皮下注射。停药后第7天将大鼠麻醉，立体定向引导下将刺激电极置入单侧NAc，在刺激前后用微透析探针对同侧NAc，VP和VTA行微透析，用高效液相色谱法（high performance liquid chromatography，HPLC）测定透析液中的Glu和GABA的含量。对照组大鼠只植入电极，不刺激。结果吗啡组NAc中Glu含量增高。对NAc行高频电刺激后，NAc和VTA中细胞外Glu含量递减，GABA含量无明显变化；VP中细胞外Glu和GABA含量无明显变化。结论 Glu在维持吗啡精神依赖的过程中发挥重要作用，高频电刺激能通过减少NAc和VTA中细胞外Glu的含量来消除吗啡精神依赖。

关键词: 吗啡, 精神依赖, 伏隔核, 高频电刺激, 谷氨酸

Abstract:

Objective To investigate the changes of levels of glutamate(Glu) and gammaaminobutyric acid(GABA) in nucleus accumbens(NAc), ventral pallidum(VP) and ventral tegmental area(VTA) after high frequency electrical stimulation of NAc in morphine psychic dependent rats, and illustrate the mechanism of high frequency electrical stimulation treatment of drug psychic dependence. Methods Morphine hydrochloride was injected subcutaneously in Wistar rats for 10 days(3 times per day), sodium chloride solution was injected subcutaneously in rats of control group. At the 7th day after withdrawal of morphine, Concentric bipolar electrodes were stereotaxically implanted into the NAc and microdialysis probes were unilaterally placed into NAc, VP and VTA of rats after anesthesia, the microdialysates before stimulation and after stimulation were collected. The levels of extracellular Glu and GABA in microdialysates were determined by high-performance liquid chromatography(HPLC). In control group, only stimulating electrodes were implanted, stimulation was not proceeded.Results The levels of extracellular Glu in NAc increased in morphine group. During and after stimulation of NAc, the levels of extracellular Glu in NAc and VTA decreased, but the levels of extracellular GABA did not change; The levels of extracellular Glu and GABA in VP did not change. Conclusion Glu is an important factor to maintain psychic dependence, and high frequency electrical stimulation can induce decrease in extracellular Glu levels in NAc and VTA to diminish morphine psychic dependence.

Key words: morphine, psychic dependence, nucleus accumbens, high frequency electrical stimulation, glutamate

中图分类号:

R 74

胡文瀚;张颖;孟凡刚;张斌;张凯;张建国. 高频电刺激吗啡精神依赖大鼠伏隔核后相关脑区细胞外氨基酸类神经递质的变化[J]. 首都医科大学学报, 2010, 31(5): 600-603.

HU Wen-han;ZHANG Ying;MENG Fan-gang;ZHANG Bin;ZHANG Kai;ZHANG Jian-guo. Changes of Extracellular Amino Acids Levels in Related Brain Regions after High Frequency Electrical Stimulation of Nucleus Accumbens in Morphine Psychic Dependent Rats[J]. Journal of Capital Medical University, 2010, 31(5): 600-603.

[1]	贾竑晓;何淑嫦;唐永怡;江镇康;张继志. 健脾补肾方对小鼠记忆障碍模型的影响[J]. 首都医科大学学报, 2009, 30(1): 24-26.
[2]	岳剑宁. 舒芬太尼与吗啡预充用于剖宫产术后硬膜外镇痛的比较[J]. 首都医科大学学报, 2009, 30(1): 107-109.
[3]	康凯;徐铭军. 吗啡鞘内超前镇痛对剖宫产术后镇痛的影响[J]. 首都医科大学学报, 2005, 26(5): 634-636.
[4]	刘冬梅;杨荪;周以健;王蓬文;王兴翠. 谷氨酸单钠对大鼠睾丸的影响[J]. 首都医科大学学报, 1998, 19(4): 319-322.
[5]	鲁晓莉;高清玲;丁育基;陈文东;李雅莉;李林. 氯化钾对神经母细胞瘤细胞系谷氨酸释放的影响[J]. 首都医科大学学报, 1998, 19(3): 246-249.
[6]	刘冬梅;杨荪;王蓬文;周以健. 谷氨酸单钠对大鼠垂体促性腺激素细胞的影响[J]. 首都医科大学学报, 1998, 19(3): 213-216.
[7]	王秀琴;曾晓蓓;米娜娃;杨荪;王蓬文. 谷氨酸单钠致大鼠肾上腺组织化学变化的研究[J]. 首都医科大学学报, 1998, 19(2): 109-112.
[8]	李玉兰;李郁英;安云庆;杨佩荪;刘冬梅. 谷氨酸单钠对大鼠发育和免疫功能的影响[J]. 首都医科大学学报, 1997, 18(3): 218-221.
[9]	王蓬文;杨佩荪;刘国贞;汪红焱. 谷氨酸单钠对大鼠垂体生长激素细胞发育的影响[J]. 首都医科大学学报, 1997, 18(3): 214-217.
[10]	鲁厚祯;王卫;郎晓燕;杨连雪;杨佩荪;王蓬文. 谷氨酸钠致使新生大鼠海马锥体细胞损伤的形态学研究[J]. 首都医科大学学报, 1996, 17(3): 168-172.
[11]	杨珮荪;林锴;王蓬文;刘国贞. 谷氨酸单钠对大鼠骨发育的影响[J]. 首都医科大学学报, 1994, 15(3): 184-187.
[12]	杨珮荪;林锴;盛树力;高林. 谷氨酸单钠引起大鼠垂体性侏儒变化[J]. 首都医科大学学报, 1990, 11(4): 283-288.