正常人和肝癌患者血清β-葡萄糖醛酸苷酶活性测定

首都医科大学学报 ›› 1984, Vol. 5 ›› Issue (1): 48-50.

正常人和肝癌患者血清β-葡萄糖醛酸苷酶活性测定

袁振铎

北京友谊医院北京市临床医学研究所生化研究室肿瘤组

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1984-01-15 发布日期:1984-01-15

A Method for the Determination of Serum β-Glucuronidase Activity in Healthy Personnel and Hepatoma Patients

Yuan Chenduo

Received:1900-01-01 Revised:1900-01-01 Online:1984-01-15 Published:1984-01-15

摘要/Abstract

摘要： 本文介绍了一种血清β-萄葡糖醛酸苷酶活性测定法。反应混合物在37℃保温18小时,测定与酶浓度呈函数关系的基质的水解作用,是在560nM波长下进行的。测定了正常人91例,平均值378±142U%(±S.D),正常上限定为600U%。肝癌患者29例,其平均值为984±592U%(±S.D),明显增高。阳性率为76%二者有非常显著差异(p<0.001)。

Abstract: A colorimetric method for determining the serum β-glucuronidase activity was reported. The mixture was incubated at 37℃ for 18 hr, the degree of hydrolysis of the substrate as a function of enzyme activity was determined by a Model 72 spectrophotometer at the wavelength of 560 nM. The mean serum enzyme activity of 91 healthy blood donors and volunteers was 378 + 142 U% (X±S.D.).The upper limitfor normal range was arbitrarily taken as 600U%. Serum β-glucuronidase activity was markedly increased in 29 patients with hepatic cancer, in which the mean serum enzyme activity was 984±592U%(X ± S.D.) and 22(76%)out of them were abnormal (p<0.001).

袁振铎. 正常人和肝癌患者血清β-葡萄糖醛酸苷酶活性测定[J]. 首都医科大学学报, 1984, 5(1): 48-50.

Yuan Chenduo. A Method for the Determination of Serum β-Glucuronidase Activity in Healthy Personnel and Hepatoma Patients[J]. Journal of Capital Medical University, 1984, 5(1): 48-50.