用凝胶色谱法研究α,α′-联硫二[β-(2-呋喃)丙烯酸]对锌和人血清清蛋白结合的影响

首都医科大学学报 ›› 1986, Vol. 7 ›› Issue (4): 267-271,333.

用凝胶色谱法研究α,α′-联硫二[β-(2-呋喃)丙烯酸]对锌和人血清清蛋白结合的影响

曾昭晖, 张锦楠, 阎淑莲

北京第二医学院化学教研室

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1986-10-15 发布日期:1986-10-15

Studies of the Effect of α,α'-dithiobis [β-2-furan)acrylic acid] on Binding of Zinc to HSA by Gel Chromatography

Zeng Zhaohui

Department of Chemistry

Received:1900-01-01 Revised:1900-01-01 Online:1986-10-15 Published:1986-10-15

摘要/Abstract

摘要：

用凝胶色谱法研究了α,α′-联硫二[β-(2-呋喃)丙烯酸](DTFA)对HSA—Zn 结合的影响,实验证明DTFA 能提高HSA—Zn 的结合量。HSA—Zn 的结合百分数由HSA—Zn 体系中的38.28%增加到HSA—Zn—DTFA 体系中的66.40%。最适摩尔比约为Zn∶DTFA=2∶1。

Abstract:

In this paper,the effect of α,α′-dithiobis[β-(2-furan)acrylicacid](DTFA)on binding Zn to HSA by gel chromatography wasstudied.The amounts of HSA bound Zn,the molar ratio of Zn to DTFAand fluorescence spectrum of HSA were determind.It was found that:1.The DTFA could markedly increase the amount of HSA boundZn.In systems of HSA,Zn and DTFA the amount of Zn-HSA was 66.40%and the amount of Zn-HSA only 38.28% in the systems of Zn and HSA.The results obtained in the present investigation were consistent withthe animal experiment.2.The principal peak in the fluorescence spectrum of HSA was 345nm on excitation wave length 295 nm.Asimilarity in wave length of thefluorescence peak of HSA-Zn-DTFA systems was observed.Theseobservations suggestsd that mechanism of DTFA induced increase inserum zinc concentration involved the formation of a ternary complex ofAlbumin,Zinc and DTFA.

曾昭晖;张锦楠;阎淑莲. 用凝胶色谱法研究α,α′-联硫二[β-(2-呋喃)丙烯酸]对锌和人血清清蛋白结合的影响[J]. 首都医科大学学报, 1986, 7(4): 267-271,333.

Zeng Zhaohui. Studies of the Effect of α,α'-dithiobis [β-2-furan)acrylic acid] on Binding of Zinc to HSA by Gel Chromatography[J]. Journal of Capital Medical University, 1986, 7(4): 267-271,333.