肺吸虫成虫抗原组分分析

首都医科大学学报 ›› 1991, Vol. 12 ›› Issue (4): 293-295.

肺吸虫成虫抗原组分分析

张月清¹, 桂淑环¹, 吴赵永¹, 杨绍华², 王泽生²

1. 北京热带医学研究所;2. 首都医学院

收稿日期:1990-06-08 修回日期:1900-01-01 出版日期:1991-10-15 发布日期:1991-10-15

Studies of the Antigenic Components of Paragonimus Westermani

Zhang Yueqing¹, Gui Shuhuan¹, Wu Zhaoyong¹, Yang Shaohua², Wang Zesheng²

1. Beijing Tropical Medicine Research Institute;2. Laboratory Center and Department of Biochemistry, Capital Institute of Medicine

Received:1990-06-08 Revised:1900-01-01 Online:1991-10-15 Published:1991-10-15

摘要/Abstract

摘要： 用IE、SDS-PAGE和Western blot对肺吸虫成虫抗原(PwA)成分进行分析.结果IE出现2条粗而明显的沉淀带。SDS-PAGE显示29条蛋白带(分子量范围为90.0～6.0kD),其中有10条主带,分子量分别为57、53、49.5、46、42、30、27、25、14和13 kD.经Western blot试验显示,肺吸虫病人血清(抗体)与PwA应答,可出现3条蛋白带,分子量分别为25、13和8 kD。

关键词: 肺吸虫抗原, 免疫电泳, 十二烷基硫酸钠-聚丙烯酰胺凝胶电泳, 免疫印迹

Abstract: The components of soluble antigen of Paragonimus vestermani(PwA)were analysed with immuno-electrophoresis(IE),Sodium dodecyl sulfate-polyacrylamide gel eleetrophoresis (SDS-PAGE),and western blot.Two broad and distinct bands only were detected in IE. 29 protein bands were found to be present when adult worm homogenates were subjected to SDS-PAGE analysis,and their molecular weights ranged from 90.0～6.0 kD.The molecular weight of the 10 most prominent protein bands was corresponeded to 57,53,49.5,46, 42,30,27,25,14 and 13 kD respectively.The western blot examination of PwA with sera of proven cases of paragonimiasis revealed 3 bands,whose protein molecular weights were 25, 13,and 8 kD respectively.

Key words: paragonimus westermani, component analysis, immuno-electrophoresis(IE), sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE), western blot

张月清;桂淑环;吴赵永;杨绍华;王泽生. 肺吸虫成虫抗原组分分析[J]. 首都医科大学学报, 1991, 12(4): 293-295.

Zhang Yueqing;Gui Shuhuan;Wu Zhaoyong;Yang Shaohua;Wang Zesheng. Studies of the Antigenic Components of Paragonimus Westermani[J]. Journal of Capital Medical University, 1991, 12(4): 293-295.

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