早期心肌缺血的病理学诊断方法探讨肌酸激酶的免疫组化染色法

首都医科大学学报 ›› 1995, Vol. 16 ›› Issue (1): 1-6.

早期心肌缺血的病理学诊断方法探讨肌酸激酶的免疫组化染色法

李文, 温祥云, 陈铁军, 周以健

首都医学院病理解剖学教研室

收稿日期:1993-10-23 修回日期:1900-01-01 出版日期:1995-01-15 发布日期:1995-01-15

An Approach to the Pathologic Diagnosis of Early Myocardial Ischemia ──immuno-histochemical staining method for creatine kinase

Li Wen, Wen Xiangyun, Chen Tiejun, Zhou Yijian

Department of Pathology, Capital Inuitute of Medicine

Received:1993-10-23 Revised:1900-01-01 Online:1995-01-15 Published:1995-01-15

摘要/Abstract

摘要：

对实验动物的缺血心肌进行PAP法染色,结果心肌缺血40min后,着色即有变化,并随缺血时间的延长而逐渐明显,证实肌酸激酶的免疫组化染色是一种早期诊断心肌缺血的特异性好、敏感性高的重要辅助方法。

关键词: 心肌缺血, 肌酸激酶, 免疫组织化学染色

Abstract:

Pathologic diagnosis of early myocardial ischemia is a very important problem. We tried to research the specificity and sensitivity of creatine kinase immunohistochemical stain for the diagnosis of early myocardial ischemia. At first,the pure antigen was extracted from the skeletal muscles of SD rats. The rabbit was immunized and anti-rat antibody was obtained.The left coronary artery of female SDrat was ligated in order to make the myocardium ischemic 20, 40, 60, 120 and 140 min after ligation, the heart was taken out, the myocasdial specimens were embeded in paraffin and their sections were stained according to PAP method. The results showed that discolouration of the ischemic myocardium accurred if ischemic time was more than 40 min. So,we conclude that the creatine kinase PAPstaining method is an effective diagnostic method and has higher specificity and sensitivity for early myocardial ischemia.

Key words: myocardial ischemia, creatine kinase, immunohistochemical staining

中图分类号:

R362

李文;温祥云;陈铁军;周以健. 早期心肌缺血的病理学诊断方法探讨肌酸激酶的免疫组化染色法[J]. 首都医科大学学报, 1995, 16(1): 1-6.

Li Wen;Wen Xiangyun;Chen Tiejun;Zhou Yijian. An Approach to the Pathologic Diagnosis of Early Myocardial Ischemia ──immuno-histochemical staining method for creatine kinase[J]. Journal of Capital Medical University, 1995, 16(1): 1-6.

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