关键词: 阳离子脂质体, 原代骨胳肌细胞, LacZ基因, 转基因

Abstract:

Two kinds of cationic liposomes, lipofectin and lipofectaMINE, were used for transfecting reporter gene LacZ into primary cultural rat muscle cells.The cells were prepared by trypsinization of new-born SD rat musele.The reporter gene was the gene ofβ-galactosidase (LacZ) and from a plasmid, pRSVLacZ.The results showed that both lipofectin and lipofecta MINE could transfer exogenous DNA of LacZ into cultured muscle cells which expressed the reporter gene by 20%.This suggested that the transfection method by using cationic liposome was quite effective for gene transfer into eukaryotic cells.

Key words: cationic liposome, primary cultural muscle cells, LacZ gene, gene transfer