急性重复性缺氧小鼠脑组织中儿茶酚胺类神经递质含量变化的研究

首都医科大学学报 ›› 1995, Vol. 16 ›› Issue (3): 185-188.

急性重复性缺氧小鼠脑组织中儿茶酚胺类神经递质含量变化的研究

黄如彬, 杨典洱, 潘颖, 李爱华

首都医学院生化教研室

收稿日期:1994-04-20 修回日期:1900-01-01 出版日期:1995-07-15 发布日期:1995-07-15

Studies of Catecholamine Transmitters in Acute Repetitive Hypoxia of Mouse Brain

Huang Rubin, Yang Dianer, Pan Ying, Li Aihua

Department of Biochemistry, Capital Institute of Medicine

Received:1994-04-20 Revised:1900-01-01 Online:1995-07-15 Published:1995-07-15

摘要/Abstract

摘要： 用高效液相-电化学检测器测定急性重复性缺氧小鼠脑组织中DOPA、DA、NE和Ep等儿茶酚胺类物质的含量。结果发现:与空白对照组(A组)比较,缺氧1次的实验对照组(B组)及急性重复缺氧4次实验组(C组),随缺氧次数增加,小鼠脑组织中的DA含量显着上升(P<0.01),NE、DOPA含量明显降低(P<0.05或<0.01);A组与急性重复4次缺氧后饲养2d的实验对照组(D组)小鼠脑组织中儿茶酚胺类含量接近,没有统计学意义(P=23.77).结果提示脑组织中儿茶酚胺类物质可能参与急性重复缺氧小鼠的耐缺氧能力的形成,而且是一个可逆的快反应。

关键词: 重复缺氧, 儿茶酚胺类, 高效液相色谱分析

Abstract: Kunming mouse was chosen as the experimental animal. The levels of DOPA,DA,NE and EP in brain tissue were measured by high-performance liquid chromatography under acute repetitive hypoxia. As compared with the group Aof blank control without exposed to hypoxia ,group B of experimental control was exposured to hypoxia only once ,and group D of another experimental control was exposed acutely and repetitively to hypoxia for 4 times and then fed for 2 days, the levels of DAincreased significantly and that of DOPA,NE decreased markedly in experimental animals, group C ,acutely and repetitively exposed to hypoxia for 4 times; while the levels of catecholamines were similar between group A and D. There was no significant differences. The results indicated that catecholamines in brain might be involved in the development of tolerance in animals exposed to acute and repetitive hypoxia ,and the process might be a fast reversible response.

Key words: repetitive hypoxia, catecholamines, HPLC

中图分类号:

R593.2

黄如彬;杨典洱;潘颖;李爱华. 急性重复性缺氧小鼠脑组织中儿茶酚胺类神经递质含量变化的研究[J]. 首都医科大学学报, 1995, 16(3): 185-188.

Huang Rubin;Yang Dianer;Pan Ying;Li Aihua. Studies of Catecholamine Transmitters in Acute Repetitive Hypoxia of Mouse Brain[J]. Journal of Capital Medical University, 1995, 16(3): 185-188.

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