蜂窝哈夫尼亚菌脂多糖核心区的免疫学特征

首都医科大学学报 ›› 1995, Vol. 16 ›› Issue (3): 189-193.

蜂窝哈夫尼亚菌脂多糖核心区的免疫学特征

安云庆, Appelmelk B J

1. 首都医学院微生物学教研室;2. 荷兰自由大学医学微生物学教研室

收稿日期:1995-01-04 修回日期:1900-01-01 出版日期:1995-07-15 发布日期:1995-07-15

Immunological Characteriotics of Lipopolysaccharide Core Region of Hafnia Alvei

An Yunqing, Appelmelk B J

1. Department of Medical Microbiolegy and Immunology, Capital Institute of Medicine;2. Department of Medical Microbiology of Free University, The Netherlands

Received:1995-01-04 Revised:1900-01-01 Online:1995-07-15 Published:1995-07-15

摘要/Abstract

摘要： 用单克隆抗体和多克隆抗血清对8株Hafniaalvei(H.alvei)的脂多糖核心区进行了测定,结果如下:①用KDO-特异性单克隆抗体(克隆14和20)分别在6株和5株H.alvei的脂多糖中检测到α-连接的KDO侧链,②E.coli核心型(R₁～R₄)特异性单克隆抗体和吸收后单一特异性抗血清均不能与H.alvei的脂多糖核心发生反应。③E.coli-Rc(化学型)核心特异性单克隆抗体(WN1222-5)能与所有(8株)测试的H.alvei的脂多糖发生反应。结果表明,H.alvei含有类似于E.coli-KDO侧链和Rc(化学型)核心的抗原结构,但两者己糖区核心型(R₁～R₄)抗原不同。

关键词: 蜂窝哈夫尼亚菌, 脂多糖核心, 单克隆抗体

Abstract: Monoclonal antibodies(Mabs)and polyclonal antisera (Pabs) were used for characterizing the LP Score of 8 H.alvei strains :①Two anti-KDO Mabs (clone 14,20) detected α-linked KDO side chain in several(6, 5) but not all of the LPS. ②Mabs and Pabs specific for the R₁～R₄ core of E.coli did not react to the LPS core of H.alvei. ③The Mab (WN1222-5) specific for the Rc chemistry core of E.coli reacted strongly with all LPS of H.alvei tested. We conclude that the LPS of H.alvei contains both eiptopes (α-linked KDO side chain,Rc corel similar to those occurring in E.coli, but they are different in the core (R₁～R₄)types.

Key words: Hafnia alvei, lipopolysaccharide core (LPS core), monoclonal antibodies (Mabs)

中图分类号:

R378.2

安云庆;Appelmelk B J. 蜂窝哈夫尼亚菌脂多糖核心区的免疫学特征[J]. 首都医科大学学报, 1995, 16(3): 189-193.

An Yunqing;Appelmelk B J. Immunological Characteriotics of Lipopolysaccharide Core Region of Hafnia Alvei[J]. Journal of Capital Medical University, 1995, 16(3): 189-193.

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