高效液相色谱法分离测定鱼油中的EPA和DHA

首都医科大学学报 ›› 1995, Vol. 16 ›› Issue (4): 263-267.

高效液相色谱法分离测定鱼油中的EPA和DHA

郭爱民¹, 王晖¹, 尹娜¹, 张淑华¹, 燕福生²

1. 首都医学院卫生化学教研室;2. 首都医学院化学教研室

收稿日期:1994-12-27 修回日期:1900-01-01 出版日期:1995-10-15 发布日期:1995-10-15

Separation and Determination of Eicosapentaenoic Acid and Docosahexaenoic Acid in Fish Oil by High Performance Liquid Chromatography

Guo Aimin¹, Wang Hui¹, Yin Na¹, Zhang Shuhua¹, Yan Fusheng²

Department of Public Health, Capital Institute of Medicine

Received:1994-12-27 Revised:1900-01-01 Online:1995-10-15 Published:1995-10-15

摘要/Abstract

摘要： 介绍用高效液相色谱法分离测定鱼鱼油及其制品中的二十碳五烯酸(eicosapentaenoic acid,EPA)和二十二碳六烯酸(docosahexaenoic acid,DHA)方法。使用Waters液相色谱仪,μ-Bondpark C₁₈H₃₇填充柱(30cm×4mm).鱼油经0.5mol/LKOH乙醇溶液皂化,三乙胺-ω-溴苯乙酮酯化后直接进样。以88%的甲醇水溶液为流动相,紫外254nm检测,用外标法测定。该方法的回收率EPA为93.0%～98.0%,DHA为93.0%～100.0%.相对标准偏差EPA为0.41%～0.86%,DHA为0.67%～1.20%(n=7).本法简便、快速、重现性好。

关键词: EPA, DHA, 鱼油, 高效液相色谱法

Abstract: The method of high performance liquid chromatography analysis for eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in the anchovy oil was reported.The sample was determined with HPLC system of model Waters and μ-Bondpark C₁₈ colume (30cm×4 mm i.d).The EPAand DHA in fish oil was saponified with 0.5 mol/L KOH-C₂H₅OH,esterified with triethylamine-ω-Bromoacetopnenone, and inject directly into the system of HPLC.UVdetection was performed at 254 nm,with the mobile phase of Methanol-water(88:12).The contents of the EPA and DHA in the sample were determined by standard curve.The recovery rates of EPA and DHA were 93.0%～98.0% and 93.0%～100.0% respectively.The relative standard deviations of EPA and DHA were 0.41%～0.86% and 0.67%～1.20% (n=7).This method is simpie,rapid and with good repeatability.

Key words: EPA: DHA, fish oil, HPLC

中图分类号:

O657.72

郭爱民;王晖;尹娜;张淑华;燕福生. 高效液相色谱法分离测定鱼油中的EPA和DHA[J]. 首都医科大学学报, 1995, 16(4): 263-267.

Guo Aimin;Wang Hui;Yin Na;Zhang Shuhua;Yan Fusheng. Separation and Determination of Eicosapentaenoic Acid and Docosahexaenoic Acid in Fish Oil by High Performance Liquid Chromatography[J]. Journal of Capital Medical University, 1995, 16(4): 263-267.

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