4株蓝氏贾第虫总细胞蛋白质等电聚焦电泳分析

首都医科大学学报 ›› 1995, Vol. 16 ›› Issue (4): 268-270.

4株蓝氏贾第虫总细胞蛋白质等电聚焦电泳分析

刘华¹, 卢思奇², 王凤芸², 王泽生¹

1. 首都医学院生物化学教研室;2. 首都医学院寄生虫学教研室

收稿日期:1995-01-14 修回日期:1900-01-01 出版日期:1995-10-15 发布日期:1995-10-15

Isoelectric Focusing of Total Cell Proteins of Four Giardia lamblia Isolated from China and America

Liu Hua¹, Lu Siqi², Wang Fengyun², Wang Zesheng¹

Department of Biochemistry, Capital Institute of Medicine

Received:1995-01-14 Revised:1900-01-01 Online:1995-10-15 Published:1995-10-15

摘要/Abstract

摘要： 用薄层等电聚焦电泳法,对分离自我国北京、四川、福建以及美国的4株蓝氏贾第虫总细胞蛋白质做了分析。结果表明,国内3株的带型基本一致,各带分布于标准pI3.6～8.3之间;美国1株的带型与国内3株的不同,多出2条区带,分别位于pI7.8和7.1处。提示中美虫株在蛋白质组成成分上存在差异,其原因可能系地理分隔所致。

关键词: 蓝氏贾第虫, 总细胞蛋白质, 等电聚焦电泳

Abstract: The total cell proteins obtained from the trophozoites of four Giardia lamblia isolates, of which three were isolated from China (C₁, C₂, and C₃ ), and one from America(CDC), were studied by using an isoelectric focusing technique.It was shown that each of the three domestic isolates produced identical banding patterns,of which bands rang from pl 3.6 to 8.3.The banding pattern of the Anierican isolate was different from those of the Chinese isolates,which produced two adclitional new bands.The two distuict bands located at pI 7.1 and 7.8 respectively.The present study shows that geographical separation may be one of the reasons which causes difference between Giardia isolates from different geographical regions.

Key words: Giardia lamblia, total cell proteins, isoelectric focusing

中图分类号:

R382

刘华;卢思奇;王凤芸;王泽生. 4株蓝氏贾第虫总细胞蛋白质等电聚焦电泳分析[J]. 首都医科大学学报, 1995, 16(4): 268-270.

Liu Hua;Lu Siqi;Wang Fengyun;Wang Zesheng. Isoelectric Focusing of Total Cell Proteins of Four Giardia lamblia Isolated from China and America[J]. Journal of Capital Medical University, 1995, 16(4): 268-270.