间羟苯甲酸4-羟化酶的化学计量学研究

首都医科大学学报 ›› 1997, Vol. 18 ›› Issue (2): 110-113.

间羟苯甲酸4-羟化酶的化学计量学研究

陈瑞¹, 细川桂一², 黄如彬¹, 赵慧芳¹

1. 首都医科大学生物化学教研室;2. 日本东京バイオ微生物研究所

收稿日期:1997-01-24 修回日期:1900-01-01 出版日期:1997-04-15 发布日期:1997-04-15

Stoichiometry of meta-Hydroxybenzoate 4-Hydroxylase

Chen Rui¹, Keiichi Hosokawa², Huang Rubin¹, Zhao Huifang¹

1. Department of Biochemistry, Capital University of Medical Sciences;2. Institute of Bio-Microbiology, Tokyo Japan

Received:1997-01-24 Revised:1900-01-01 Online:1997-04-15 Published:1997-04-15

摘要/Abstract

摘要： 用扫描分光光度计对酶所催化的反应进行化学计量学研究.在反应开始后的不同时间,在200～400nm波长范围内扫描,观察到不同的吸收波谱,直到吸收波谱不再改变为止,由此得到间羟苯甲酸4羟化酶催化间羟苯甲酸转变成3,4二羟苯甲酸随时间变化的吸收波谱.用摩尔消光系数计算每种成分的浓度,在2次独立的实验中,底物与产物的摩尔比值分别为1:0.998和1:1.085,提示来源于Co. testosteroniKH1223S菌株的MOB4HOase与文献报道的来源于Aspergilusniger菌株的MOB4HOase所催化的反应是相同的.

关键词: 间羟苯甲酸4-羟化酶, 化学计量学, 吸收波谱, 丛毛单孢菌

Abstract: Stoichiometry of catalytic reaction by enzyme can be researched by scanning spectrophotometry. The difference absorption spectra at wave lengths between 200 and 400 nm were observed after starting the reaction at different time until no further change in the absorption profile could be detected.Therefore it was obtained that the absorption spectra of conversion of m hydroxybenzoate to protocatechuate by meta hydroxybenzoate 4-hydroxylase changed following the time. Concentration of each component was calculated from the molar extinction coefficient. The molar ratioes of substrate to product in two independent experiments were 1:0.998 and 1:1.085 respectively,indicating a good accord to the reaction by the MOB4 HO ase extracting from Co. testosteroni KH122-3S and Aspergillus niger genera.

Key words: meta hydroxybenzoate 4-hydroxylase, stoichiometry, absorption spectrum, Comamonas testosteroni 

中图分类号:

Q554.7

陈瑞;细川桂一;黄如彬;赵慧芳. 间羟苯甲酸4-羟化酶的化学计量学研究[J]. 首都医科大学学报, 1997, 18(2): 110-113.

Chen Rui;Keiichi Hosokawa;Huang Rubin;Zhao Huifang. Stoichiometry of meta-Hydroxybenzoate 4-Hydroxylase[J]. Journal of Capital Medical University, 1997, 18(2): 110-113.