植物血凝素激活的LAK细胞激活培养条件与其生物学特性关系的研究

首都医科大学学报 ›› 1997, Vol. 18 ›› Issue (2): 105-109.

植物血凝素激活的LAK细胞激活培养条件与其生物学特性关系的研究

靖学芳

首都医科大学微生物学教研室

收稿日期:1996-06-05 修回日期:1900-01-01 出版日期:1997-04-15 发布日期:1997-04-15

Study on the Relationship between the Preming and(or)Cultivating Condition of Human PHA-LAK Cells and their Biological Charateristics

Jing Xuefang

Department of Microbiology, Capital University of Medical Sciences

Received:1996-06-05 Revised:1900-01-01 Online:1997-04-15 Published:1997-04-15

摘要/Abstract

摘要： 将PHA和rIL2合理配伍应用于LAK细胞的激活培养中,研究了PHA-LAK细胞的增殖、细胞毒活性及表型.结果表明:培养条件中加入适量PHA可促进PHA-LAK的增殖(P<0.05),不影响其细胞毒活性.PHA/rIL-2共同预刺激组于培养第2d细胞数明显下降(30%～40%),但其到达增殖高峰的时间、峰值及扩增倍数与同一培养条件下的PHA单独预刺激组相比,差别无显着性(均为P>0.05);但前者对K562和Raji的杀伤高峰值大于后者(P<0.01).各组PHA-LAK中有0.60～0.77的细胞为CD3⁺T细胞,但PHA-LAK/rIL-2共同预刺激者的CD3⁺、CD8⁺细胞所占比率较PHA单独预刺激者为高(P<0.05).

关键词: HA-LAK细胞, 增殖, 细胞毒活性, 表型

Abstract: Some biological charateristics(proliferation response, cytotoxicity,phenotype)of PHA-LAKcells under different preming and cultivating conditions were studied. The proliferation responses showed that the number of PHA-LAKcells after co priming for 48 h with PHA/rIL-2 declined dramatically during the first two days, and reached the peak value of proliferation on the same day(day 6 after priming)as did those PHA-LAKcells primed with PHAalone under the same cultivating condition, and the differences in the peak value and multiplication fold on every same day between the PHApreming alone and the PHA-LAKco priming group were statistically insignificant(P>0.05). The cytotoxicity results showed that the peak time of cytotoxicity of PHA-LAKcells co primed with PHA/rIL-2 against K₅₆₂ and Raji cell reached on day 6 after co priming, two days earlier than that of PHA-LAKcells premied with PHAalone and the peak value of the former against Raji cells was higher than that of the latter(P<0.01). The phenotype analysis showed that about 0.60～0.77 of PHA-LAKcells were CD3⁺ cells under different priming and cultivating condition, and CD3⁺,CD8⁺ cells of PHA-LAKprimed with PHA/rIL-2 were more than those of PHA-LAKprimed with PHAalone(P<0.05).

Key words: PHA-LAK cells, proliferation, cytotoxicity, phenotype

中图分类号:

R392.11

靖学芳. 植物血凝素激活的LAK细胞激活培养条件与其生物学特性关系的研究[J]. 首都医科大学学报, 1997, 18(2): 105-109.

Jing Xuefang . Study on the Relationship between the Preming and(or)Cultivating Condition of Human PHA-LAK Cells and their Biological Charateristics[J]. Journal of Capital Medical University, 1997, 18(2): 105-109.

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