应用BiostarH-Ⅰ型基因芯片筛选肺鳞癌中细胞信号传导及免疫相关基因

首都医科大学学报 ›› 2005, Vol. 26 ›› Issue (6): 729-732.

应用BiostarH-Ⅰ型基因芯片筛选肺鳞癌中细胞信号传导及免疫相关基因

白澎, 张沪生, 王毓洲

首都医科大学附属北京同仁医院呼吸内科

收稿日期:2004-08-31 修回日期:1900-01-01 出版日期:2005-12-24 发布日期:2005-12-24

Screening Genes Associated with Biological Signal Conduction and Immunity in Lung Squamous Cell Carcinoma by BiostarH-Ⅰ cDNA Microarray

Bai Peng, Zhang Husheng, Wang Yuzhou

Department of Respiratory Disease, Beijing Tongren Hospital, Capital University of Medical Sciences

Received:2004-08-31 Revised:1900-01-01 Online:2005-12-24 Published:2005-12-24

摘要/Abstract

摘要： 目的利用基因芯片技术,寻找人类肺鳞癌组织和正常肺组织中与生物信号传导或免疫反应有关的差异表达基因。方法提取肺鳞癌和正常肺组织总RNA,反转录合成cDNA探针,混合后与BiostarH-Ⅰ型基因芯片杂交,ScanArray4000扫描仪扫描杂交信号,计算机分析差异表达基因。结果筛选出差异表达的已知基因8条,其中5条表达上调(r>2.0),分别为METAP2、LIV-1、CUL2、CSE1L、RBBP2H1A;3条表达下调(r<0.5),分别为CX3CL1、HBB、CKB。结论利用基因芯片技术实现高通量筛选肺癌发病相关基因,发现8条在肺鳞癌中与细胞信号传导或免疫反应有关的差异表达基因,为今后更深入的研究指明了方向。

关键词: 微矩阵基因芯片, 肺肿瘤, 免疫, 生物信号传导

Abstract: Objective By use of cDNAmicroarray, to screen out the differential expression genes which are related with biological signal conduction, humoral or cellular immunity in lung squamous cell carcinoma and normal lung tissue.Methods The total RNAs were isolated from tumor and normal lung tissue and were reversely transcribed to prepare the hybridization probe.The mixed probes were hybridized with the BiostarH-Ⅰ cDNAmicroarray.After washing, the cDNAmicroarray was scanned for the fluorescent signals by ScanArray 4000 scaning apparatus.The scaning data was analyzed by Genepxix Pro software and differential expression genes between these two kinds of tissues were shown out.Results Eight strips of genes were screened out.Among them, five genes'expression level were up-regulated(r>2.0), including: METAP2, LIV-1, CUL2, CSE1L, RBBP2H1A, and the rest three strips were down-regulated(r<0.5), including: HBB, CKB, CX3CL1.Conclusion cDNAmicroarray can realize high-through out screening of the genes which associated with lung cancer.Eight genes which related with biological singal conduction or immunity in lung squamous cell carcinoma are found.Further investigation about these genes will be carried out later.

Key words: cDNA microarray, lung cancer, immunity, biological signal conduction

中图分类号:

R734.2

白澎;张沪生;王毓洲. 应用BiostarH-Ⅰ型基因芯片筛选肺鳞癌中细胞信号传导及免疫相关基因[J]. 首都医科大学学报, 2005, 26(6): 729-732.

Bai Peng;Zhang Husheng;Wang Yuzhou. Screening Genes Associated with Biological Signal Conduction and Immunity in Lung Squamous Cell Carcinoma by BiostarH-Ⅰ cDNA Microarray[J]. Journal of Capital Medical University, 2005, 26(6): 729-732.

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