中药黄柏的光敏抗癌作用研究

首都医科大学学报 ›› 1999, Vol. 20 ›› Issue (3): 153-155.

中药黄柏的光敏抗癌作用研究

廖静¹, 鄂征², 宁涛², 聂毓秀³, 陈玉兰³

1. 首都医科大学病理解剖学教研室;2. 北京肿瘤研究所遗传室;3. 白求恩医科大学组胚教研室

收稿日期:1998-06-19 修回日期:1900-01-01 出版日期:1999-07-15 发布日期:1999-07-15

Photosensitive Anticancer Studies on Chinese Medicine Cortex Phellodendri

Liao Jing¹, E Zheng², Ning Tao², Nie Yuxiu³, Chen Yulan³

1. Department of Pathology, Capital University of Medical Sciences;2. Beijing Institute for Cancer Research;3. Norman Bethune University of Medical Sciences

Received:1998-06-19 Revised:1900-01-01 Online:1999-07-15 Published:1999-07-15

摘要/Abstract

摘要： 以BGC823人胃癌细胞为实验材料,研究黄柏在480nm和650nm光照下对癌细胞的光敏作用。结果：黄柏加药照光组对癌细胞生长、癌细胞噻唑蓝代谢活力均有光敏抑制效应。同时,黄柏实验组癌细胞酸性磷酸酶含量明显减少(P<0.01),癌细胞³H-TdR掺入量显着降低(P<0.01),100mL/L黄柏对染色体并无光敏致粘连畸变作用,但能延缓S期细胞周期过程(P<0.01).透射电镜发现：10mL/L和100mL/L黄柏使实验组细胞线粒体、内质网广泛肿胀、扩张,细胞核糖体明显减少。提示黄柏对BGC823人胃癌细胞的确具有光敏抑制效应。

关键词: 中药黄柏, 光敏抗癌, 细胞学机制

Abstract: BGC823 human stomach cancer cells were cultured for the identification of photodynamic effects of Cortex phellodendri with illumination of 480 nm and 650 nm wave length. In the test groups with illumination, the herb extract diminished cell growth curve and the metabolism of 3-(4,5-dimethylthiazo1-2-yl)-2,5-diphenyl tetrazolium bromide(MTT). Futhermore, the significant decrease of the acid phosphatase (P<0.01) and the incorporation of ³H-TdR into cells (P<0.01) were demonstrated in the test groups.The progression of cancer cell cycle was postponed in S-phase(P<0.01) by the herb of 100 mL/L,but no chromosome adhering aberration appeared. The ultrastructural appearances showed that in the test groups, the mitochondria and the rough endoplasmic reticula of the cells became swollen and vesiculate and the ribosomes decreased.The result showed that Chineses medicine Cortex phellodendri has photosensitive effects on the cancer cells.

Key words: Chinese medicine Cortex phellodendri, photosensitive anticancer, cytological mechanism

中图分类号:

R730.52

廖静;鄂征;宁涛;聂毓秀;陈玉兰. 中药黄柏的光敏抗癌作用研究[J]. 首都医科大学学报, 1999, 20(3): 153-155.

Liao Jing;E Zheng;Ning Tao;Nie Yuxiu;Chen Yulan. Photosensitive Anticancer Studies on Chinese Medicine Cortex Phellodendri[J]. Journal of Capital Medical University, 1999, 20(3): 153-155.