人肝癌细胞和成纤维细胞共育时超微结构的改变

首都医科大学学报 ›› 2001, Vol. 22 ›› Issue (1): 10-12.

人肝癌细胞和成纤维细胞共育时超微结构的改变

张华¹, 孙海梅¹, 郭崇洁¹, 高福云², 赵天德², 魏育林²

1. 首都医科大学组织胚胎学教研室;2. 中日友好临床医学研究所细胞生物室

收稿日期:2000-01-06 修回日期:1900-01-01 出版日期:2001-01-15 发布日期:2001-01-15

Changes of the Ultrastructure of Human Hepatocellular Carcinoma Cells and Fibroblasts Cocultured in Vitro

Zhang Hua¹, Sun Haime¹, Guo Chongjie¹, Gao Fuyun², Zhao Tiande², Wei Yulin²

1. Department of Histology and Embryology, Capital University of Medical Sciences;2. Department of Cell Biology, China Japan Friendship Institute of Clinical Medical Sciences

Received:2000-01-06 Revised:1900-01-01 Online:2001-01-15 Published:2001-01-15

摘要/Abstract

摘要： 采用定位培养技术,将人肝癌细胞和成纤维细胞有序地接种在同一容器内培养,电镜下观察2种细胞接触时细胞切面超微结构的变化.结果:与肝癌细胞伪足接触的成纤维细胞膜下出现多个吞饮泡,使骨架纤维下移,局部断裂.2种细胞的线粒体肿胀,溶酶体增多,胞质中出现许多泡状结构.提示,在肝癌细胞侵袭成纤维细胞早期、成纤维细胞膜尚未受到破坏时,细胞内的结构已发生了改变.

关键词: 人肝癌细胞, 成纤维细胞, 定位培养, 超微结构

Abstract: A localized cell culture method in vitro was empolyed to investigate the interaction between human hepatocellular carcinoma cells and fibroblasts. These two kinds of cells were cocultured in the same container by design.To prepare samples for TEM, embedding slice in situ and vertical sectio ning were adopted. It was convenient to observe the ultrastructure of different kinds of cells with loca lized cell culture. The result indicated that mitochondria appeared to be swollen, the number of lysosomes increased in cytoplasm of the two kinds of cells, and microfilaments beneath the cytomembrane of fibroblasts touching the pseudopodia of the tumor cell were pushed down by pinosomes and broken at some areas after being cocultured for 96 h.The result suggests that the interaction between human hepatocellular carcinoma cells and fibroblasts causes changes of cellular structures.

Key words: human hepatocellular carcinoma cells, fibroblasts, localized cell culture, ultrastructure

中图分类号:

张华;孙海梅;郭崇洁;高福云;赵天德;魏育林. 人肝癌细胞和成纤维细胞共育时超微结构的改变[J]. 首都医科大学学报, 2001, 22(1): 10-12.

Zhang Hua;Sun Haime;Guo Chongjie;Gao Fuyun;Zhao Tiande;Wei Yulin. Changes of the Ultrastructure of Human Hepatocellular Carcinoma Cells and Fibroblasts Cocultured in Vitro[J]. Journal of Capital Medical University, 2001, 22(1): 10-12.

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