脐血CD34+细胞体外短期培养扩增研究

首都医科大学学报 ›› 2002, Vol. 23 ›› Issue (3): 218-221.

脐血CD34⁺细胞体外短期培养扩增研究

苏力, 田丁, 万岁桂, 刘聪艳, 徐娟

首都医科大学宣武医院血液科

收稿日期:2001-09-10 修回日期:1900-01-01 出版日期:2002-07-15 发布日期:2002-07-15

Study on Cord Blood CD34⁺ Cell in Short-term Culture in Vitro Amplification

Su Li, Tian Ding, Wan Suigui, Liu Congyan, Xu Juan

Department of Hematology, Xuanwu Hospital, Capital University of Medical Sciences

Received:2001-09-10 Revised:1900-01-01 Online:2002-07-15 Published:2002-07-15

摘要/Abstract

摘要： 为寻找更有效的体外扩增脐血CD34⁺细胞的造血细胞因子组合,采集健康产妇脐带血,用免疫磁珠法分选CD34⁺细胞.采用SCF、FLT3-L、TPO和IL-34种具有早期作用的细胞因子的不同组合进行脐血CD34⁺细胞短期无血清液体培养,观察培养前后有核细胞、CD34⁺细胞、CD34⁺/CD38^-细胞、CFU-GEMM、CFU-GM和BFU-E数量的变化.结果在3种不同的细胞因子组合中,同时应用SCF、FLT3-L、TPO和IL-34种细胞因子培养7d的扩增效果最好.突出的发现是在这种条件下CD34⁺/CD38^-细胞亚群达到平均197.9倍的扩增效果.提示:SCF、FLT3-L、TPO和IL-34种细胞因子是脐血CD34⁺细胞体外扩增理想的细胞因子组合.

关键词: 脐血CD34⁺细胞, 造血生长因子, CD34⁺细胞体外扩增, CD34⁺/CD38^-细胞亚群

Abstract: The objective was to explore the optimal cytokine combinations for in vitro amplification of the cord blood CD34⁺ cell. We collected the cord blood from normal full-term deliveries, and isolated CD34⁺ cells using mini MACS laboratory separation system. The purified CD34⁺cells were cultured in static short-term serum free culture system with different combinations of SCF, FLT3-L, TPO and IL-3. Result: Within the three different cytokine combinations, the combination of SCF, FLT3-L, TPO and IL-3 was proved to be the most effective, and the notable result was that the amplification of CD34⁺/CD38^- subsets reached 197.9 folds. Conclusion: The SCF, FLT3-L, TPO and IL-3 combination is the optimal cytokine combination for in vitro cord blood CD34⁺ cell amplification.

Key words: cord blood hematopoietic stem cell, progenitor cell, in vitro CD34⁺ cell amplification, CD34⁺/CD38^- subsets

中图分类号:

苏力;田丁;万岁桂;刘聪艳;徐娟. 脐血CD34⁺细胞体外短期培养扩增研究[J]. 首都医科大学学报, 2002, 23(3): 218-221.

Su Li;Tian Ding;Wan Suigui;Liu Congyan;Xu Juan . Study on Cord Blood CD34⁺ Cell in Short-term Culture in Vitro Amplification[J]. Journal of Capital Medical University, 2002, 23(3): 218-221.