心脏移植后外周血FK506结合蛋白1A基因的表达

首都医科大学学报 ›› 2002, Vol. 23 ›› Issue (2): 125-127.

心脏移植后外周血FK506结合蛋白1A基因的表达

许秀芳¹, 李温斌², 孟旭², 李文¹, 刘艳霞¹, 刘舒¹, 周健³, 黄益民¹

1. 北京市心肺血管疾病研究所细胞免疫室;2. 北京安贞医院心外科;3. 中科院微生物研究所

收稿日期:2001-10-27 修回日期:1900-01-01 出版日期:2002-04-15 发布日期:2002-04-15

Gene Expression of Peripheral Blood from a Heart Graft Patient

Xu Xiufang¹, Li Wenbin², Meng Xu², Li Wen¹, Liu Yanxia¹, Liu Shu¹, Zhou Jian³, Huang Yimin¹

1. Department of Cellular Immunology, Beijing Heart Lung and Blood Vessel Institute;2. Department of Cardiac Surgery, Beijing Anzhen Hospital;3. Microbe Graduate School of Chinese Academy cf Sciences

Received:2001-10-27 Revised:1900-01-01 Online:2002-04-15 Published:2002-04-15

摘要/Abstract

摘要： 利用荧光差异显示PCR(DD-PCR)技术检测心脏移植后外周血白细胞基因的表达。结果显示:RPL23基因移植后有波动,排斥发生时表达增强;FK506结合蛋白1A基因移植后表达较弱,排斥发生时明显增强。提示:外周血DD-PCR检测白细胞基因的表达可能对研究排斥反应机制及进行移植排斥反应诊断具有一定的意义。

关键词: 心脏移植, 荧光差异显示PCR, 差异表达, 序列测定

Abstract: In the paper the technique known as differential display polymerase chain reaction(DD-PCR)was applied to explore genes expression in white cells of peripheral blood drawn from an allogenic heart graft patient on the day before the transplant operation and after the operation in order to assess genes′role in transplantation. Morphologic changes of the heart grafts were observed 11 d after transplant operation. RPL23 gene was expressed undulatority after the operation, but strongly when acute rejection occured; FK506 binding protein 1A gene was expressed weakly after the operation,but strongly when acute rejection occured. The result indicates that the study of genes expression of peripheral blood from a heart graft patient will be useful to assess genes′role in transplantation

Key words: heart transplant|fluroDDPCR|differential expression|sequencial test

中图分类号:

R654.2

许秀芳;李温斌;孟旭;李文;刘艳霞;刘舒;周健;黄益民. 心脏移植后外周血FK506结合蛋白1A基因的表达[J]. 首都医科大学学报, 2002, 23(2): 125-127.

Xu Xiufang;Li Wenbin;Meng Xu;Li Wen;Liu Yanxia;Liu Shu;Zhou Jian;Huang Yimin. Gene Expression of Peripheral Blood from a Heart Graft Patient[J]. Journal of Capital Medical University, 2002, 23(2): 125-127.

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