腹侧海马强啡肽在癫痫敏感性增强形成中的表达

首都医科大学学报 ›› 2001, Vol. 22 ›› Issue (4): 352-354.

腹侧海马强啡肽在癫痫敏感性增强形成中的表达

王静¹, 李艺影¹, 李小勇¹, 张万琴²

1. 北京市神经外科研究所;2. 大连医科大学生理教研室

收稿日期:2000-07-12 修回日期:1900-01-01 出版日期:2001-10-15 发布日期:2001-10-15

A Expression of Dynorphin A(1-8)in Ventral Hippocampus during Induction of Enhanced Seizure Susceptibility

Wang Jing¹, Li Yiying¹, Li Xiaoyong¹, Zhang Wanqin ²

1. Beijing Neurosurgical Institute;2. Department of Physiology, Dalian Medical University

Received:2000-07-12 Revised:1900-01-01 Online:2001-10-15 Published:2001-10-15

摘要/Abstract

摘要： 用SD大鼠建立红藻氨酸(KA)诱发实验性颞叶癫痫动物模型,利用免疫细胞化学方法(ICC),研究KA诱发的癫痫敏感性增强形成过程中(KA后1～7d),大鼠腹侧海马内强啡肽^[DYNA(1-8)]免疫反应活性变化的时间过程.发现:KA后2d,大鼠腹侧海马齿状回颗粒细胞(DGC)层出现强啡肽免疫反应阳性神经细胞,KA后4d,其阳性神经细胞数量及免疫反应强度达高峰,KA后5～7d,免疫反应逐渐消失.研究表明:腹侧海马DGC层强啡肽免疫反应阳性神经细胞消失出现的时间过程(KA后5～7d)与KA诱发的癫痫敏感性增强形成出现的时间过程(KA后5～7d)一致,提示腹侧海马内源性强啡肽在抗癫痫敏感性增强形成中可能起重要作用.

关键词: 癫痫敏感性, 腹侧海马, 红藻氨酸, 强啡肽

Abstract: To study a role of dynorphin A(1-8)(DYN A(1-8))in ventral hippocampus during induction of enhanced seizure susceptibility, an acute model as human temporal lobe epilepsy was made by kainic acid(KA)causing seizure episode in the rats. The time course of alteration of dynorphin A(1-8)like immunoreactivity in ventral hippocampus during induction of enhanced seizure susceptibility(1～7 d after KA)was detected by immunocytochamical method. SD rats were given 10 mg/kg,S.C. of KA systemically. Two days later, dynorphin A(1-8)immunoreactive neurons appeared in the nuclei of dentate granule cells(DGC)in ventral hippocampus. The maximal intensity of staining and the largest number of the immunoreactive dynorphin A(1-8)occurred 4 d after KA. Five to seven days after KA, during induction of enhanced seizure susceptibility, dynorphin A(1-8)immunoreactive neurons also gradually disappeared in DGC of ventral hippocampus. Endogenous dynorphin in the seizure sensitive ventral hippocampus appeares to play an important role in regulation of brain excitability, especially in the induction of enhanced seizure susceptibility.

Key words: seizure susceptibility, ventral hippocampus, kainic acid, dynorphin A(1-8)

中图分类号:

王静;李艺影;李小勇;张万琴. 腹侧海马强啡肽在癫痫敏感性增强形成中的表达[J]. 首都医科大学学报, 2001, 22(4): 352-354.

Wang Jing;Li Yiying;Li Xiaoyong;Zhang Wanqin . A Expression of Dynorphin A(1-8)in Ventral Hippocampus during Induction of Enhanced Seizure Susceptibility[J]. Journal of Capital Medical University, 2001, 22(4): 352-354.