莪棱舌草Ⅰ号对人肝癌细胞抑制作用的研究

首都医科大学学报 ›› 2003, Vol. 24 ›› Issue (2): 100-103.

莪棱舌草Ⅰ号对人肝癌细胞抑制作用的研究

谢文杰, 刘欣, 王喆, 王娜, 付桂芳, 张瑾峰

首都医科大学细胞与遗传学教研室

收稿日期:2002-12-24 修回日期:1900-01-01 出版日期:2003-04-15 发布日期:2003-04-15

TCSs Effect of Inhibition on Human Hepatoma Cell Line 7721

Xie Wenjie, Liu Xin, Wang Zhe, Wang Na, Fu Guifang, Zhang Jinfeng

Department of Cell Biology and Genetics, Capital University of Medical Sciences

Received:2002-12-24 Revised:1900-01-01 Online:2003-04-15 Published:2003-04-15

摘要/Abstract

摘要： 运用MTT比色实验测定细胞增生能力,通过球体细胞培养、细胞生长曲线的测定以及细胞形态结构的观察,研究抗癌药莪棱舌草Ⅰ号对人肝癌细胞(7721细胞)的抑制作用。结果:莪棱舌草Ⅰ号使7721细胞的增生能力明显下降(抑制率高达70％以上),细胞球体明显减小甚至解体,细胞形态明显改变。这种抑制作用可能是莪棱舌草Ⅰ号对肝癌细胞产生杀伤作用的机制之一。

关键词: 莪棱舌草, 人肝癌细胞, 多细胞肿瘤球体, MTT测定, 生长曲线

Abstract: TCSs are compounds of anticancer medicine, including three kinds of Chinese traditional herbs. The TCSs effect of inhibition on human hepatoma cell line 7721 was determined by adopting the following four methods: MTT colorimetry experiment, multicellular tumor spheroid(MTS), measure of the cell's growth curve, and observation of the tumor cell's configuration. The results indicated that the herb obviously lowered proliferation of the tumor cell with the inhibitory rate as high as 70 %. Furthermore, the herb efficiently diminished the volume of the cell's globe or disjoint it. Finally, the cell's configuration was changed greatly by the herb. These inhibitory effects probably infer one of the mechanisms of the killing effect of TCSs on hepatoma cells.

Key words: TCSs, human liver cancer cell, multicellular tumor spheroid, MTT colorimetry, growth curve

中图分类号:

R735.7

谢文杰;刘欣;王喆;王娜;付桂芳;张瑾峰. 莪棱舌草Ⅰ号对人肝癌细胞抑制作用的研究[J]. 首都医科大学学报, 2003, 24(2): 100-103.

Xie Wenjie;Liu Xin;Wang Zhe;Wang Na;Fu Guifang;Zhang Jinfeng. TCSs Effect of Inhibition on Human Hepatoma Cell Line 7721[J]. Journal of Capital Medical University, 2003, 24(2): 100-103.

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