首都医科大学学报 ›› 2003, Vol. 24 ›› Issue (3): 228-230.

• 论著·基础研究 • 上一篇    下一篇

PMN对链球菌组蛋白样蛋白释放的影响

张力平, 张红春, 靖学芳, 沈海中, 平国铃, 李郁英   

  1. 首都医科大学微生物学教研室
  • 收稿日期:2003-02-26 修回日期:1900-01-01 出版日期:2003-07-15 发布日期:2003-07-15

Effect of Human PMN on the Streptococcal Histone-Like Protein Release

Zhang Liping, Zhang Hongchun, Jing Xuefang, Shen Haizhong, Ping Guoling, Li Yuying   

  1. Department of Microbiology, Capital University of Medical Sciences
  • Received:2003-02-26 Revised:1900-01-01 Online:2003-07-15 Published:2003-07-15

摘要: 为观察人多形核白细胞(PMN)对链球菌组蛋白样蛋白(HlpA)释放的影响,将PMN与链球菌以一定比例共同作用后取上清液做免疫印迹试验。发现与抗HlpA抗体特异性结合的蛋白条带。提示PMN作用于链球菌后,HlpA能自菌体内释放出来。

关键词: 链球菌, 多形核白细胞, 免疫印迹试验

Abstract: The objective of the study was to evaluate the effect of human PMN on Streptococcus HlpA release. Streptococcus was cultured with human PMN in different proportions and Western Blot was done to measure HlpA in the culture supernatant. There was one protein band combined specifically with anti-HlpA on Western Blot. The result indicates HlpA could be released from Streptococcus after being treated with human PMN, which is more than spontaneous release.

Key words: streptococcus, PMN, Western Blot

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