B群链球菌显色培养基在孕产妇B群链球菌筛查中的性能评价

doi:10.3969/j.issn.1006-7795.2016.05.023

首都医科大学学报 ›› 2016, Vol. 37 ›› Issue (5): 680-683.doi: 10.3969/j.issn.1006-7795.2016.05.023

B群链球菌显色培养基在孕产妇B群链球菌筛查中的性能评价

张树琛, 王杉, 朱凤霞, 时琰丽, 李东, 鲁炳怀

民航总医院检验科, 北京 100123

收稿日期:2016-03-03 出版日期:2016-10-21 发布日期:2016-10-19
通讯作者: 鲁炳怀 E-mail:zs25041@126.com
基金资助:
北京市科委首都临床特色应用研究（Z141107002514036）。

Evaluation of the performance of chromogenic agar in the screening group B streptococcus from pregnant women

Zhang Shuchen, Wang Shan, Zhu Fengxia, Shi Yanli, Li Dong, Lu Binghuai

Department of Laboratory Medicine, Civil Aviation General Hospital, Beijing 100123, China

Received:2016-03-03 Online:2016-10-21 Published:2016-10-19
Supported by:
This study was supported by Beijing Municipal Science &Technology Commission of China (Z141107002514036).

摘要/Abstract

摘要： 目的评价B群链球菌（group B streptococcus，GBS）显色培养基在孕产妇中筛查GBS的敏感性、特异性和选择性能。方法收集孕产妇肛门/阴道拭子标本共312例，已知菌株标本9例，分别直接接种或增菌后接种于血平皿和GBS显色培养基，记录鉴定结果。以筛查出的GBS总阳性数为参考，分别比较直接接种和增菌接种时，显色培养基筛查GBS的敏感性和特异性。结果显色培养基对GBS的检出率、敏感性和特异性优于常规血平皿。标本增菌后接种法孵育24 h得到的阳性率为7.05%（22/312）高于直接接种法（6.41%，20/312），对GBS筛查的敏感性高于直接接种法（95.65%vs 86.96%），特异性一致（99.65%）。结论显色培养基对GBS有良好的检出率，判断方法直观简便，对非目标菌群有良好的抑制作用。

关键词: B群链球菌, 显色培养基, 孕产妇

Abstract: Objective To evaluate the sensitivity, specificity and selective capability of chromogenic agar in the screening group B streptococcus (GBS) in the pregnant women. Methods A total of 312 rectum/vaginal swabs, collected from pregnant women, as well as 9 known GBS strains, were inoculated on the blood agar plates (BAP) and chromogenic medium chromID Strepto B agar (STRB) directly and after the selective enrichment, respectively. The screening results of the above-mentioned methods were compared. Results The STRB showed the excellent performance for GBS detection and outperformed BAP. The selective enrichment is an essential step to screen the GBS isolates due to its higher sensitivity(95.65% vs 86.96%)while both methods have the similar specificity. Conclusion STRB demonstrated good detection rate of GBS for its direct identification and effective inhibition of non-targeted bacteria.

Key words: group B streptococcus, chromogenic agar, pregnant women

中图分类号:

R446.5

张树琛, 王杉, 朱凤霞, 时琰丽, 李东, 鲁炳怀. B群链球菌显色培养基在孕产妇B群链球菌筛查中的性能评价[J]. 首都医科大学学报, 2016, 37(5): 680-683.

Zhang Shuchen, Wang Shan, Zhu Fengxia, Shi Yanli, Li Dong, Lu Binghuai. Evaluation of the performance of chromogenic agar in the screening group B streptococcus from pregnant women[J]. Journal of Capital Medical University, 2016, 37(5): 680-683.

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B群链球菌显色培养基在孕产妇B群链球菌筛查中的性能评价

Evaluation of the performance of chromogenic agar in the screening group B streptococcus from pregnant women

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