苄阿米洛利和钌红对颈动脉窦压力感受器放电的阻断作用

首都医科大学学报 ›› 2011, Vol. 32 ›› Issue (2): 218-223.

苄阿米洛利和钌红对颈动脉窦压力感受器放电的阻断作用

刘廷会¹，魏华²，赵海燕³，刘萍¹，黄海霞¹，王伟¹，付小锁¹，钮伟真^1*

1. 首都医科大学基础医学院生理学与病理生理学系； 2. 首都医科大学医学实验与测试中心；3. 首都医科大学燕京医学院生理教研室

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-04-21 发布日期:2011-04-21
通讯作者: 钮伟真

Blocking Effects of Benzamil and Ruthenium Red onCarotid Baroreceptor Discharges

LIU Ting-hui¹, WEI Hua², ZHAO Hai-yan³, LIU Ping¹, HUANG Hai-xia¹, WANG Wei¹,
FU Xiao-suo¹, NIU Wei-zhen^1*

1. Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University; 2. Medical Experiment and Test Center, Capital Medical University; 3. Department of Physiology, Yanjing Medical College, Capital Medical University

Received:1900-01-01 Revised:1900-01-01 Online:2011-04-21 Published:2011-04-21
Contact: NIU Wei-zhen

摘要/Abstract

摘要：

目的研究苄阿米洛利、阿米洛利和钌红对颈动脉窦压力感受器放电的作用，探讨压力感受器上机械敏感通道的性质。
方法制备家兔离体颈动脉窦窦神经标本，采用颈动脉窦窦内外双重灌流，钳制窦内压并记录窦神经放电，观察上述药物对压力感受器放电的作用。
结果苄阿米洛利以浓度依赖性方式阻断窦神经放电，IC50值为18.5 μmol/L，该阻断作用在冲洗后能大部分消除；阿米洛利（2 mmol/L）可阻断95%的窦神经放电，其作用可逆；钌红（100 μmol/L）以不可逆的方式抑制89%的窦神经放电活动。
结论分属A类和C类纤维的压力感受器活动均可被阿米洛利类药物和钌红阻断，提示2类压力感受纤维末梢上有相似的机械敏感通道，但是有关的机械-电转导分子还有待进一步研究。

关键词: 压力感受器, 苄阿米洛利, 钌红, 机械敏感通道, 上皮钠通道, 酸敏感通道

Abstract:

Objective To investigate the effects of benzamil, amiloride and ruthenium red(RR) on the activity of carotid sinus baroreceptor and to probe into the properties of the mechanosensitive ion channels in arterial baroreceptor.
Methods The carotid sinus nerve discharge(CSND) was recorded from rabbit carotid sinus-nerve preparations in vitro with doubly perfusing the sinus internally and externally while the intrasinus pressure was clamped. The effects of the above-mentioned chemicals on the baroreceptor discharge were investigated.
Results Benzamil blocked CSND dose-dependently with IC50=18.5 μmol/L. The effect was reversible after washing out. Amiloride(2 mmol/L) inhibited the nerve activity reversibly by 95%. RR(100 μmol/L) blocked CSND irreversibly by 89%.
Conclusion Benzamil, amiloride and RR could block the baroreceptor activities of both type A and type C nerve endings, implying that similar mechanosensitive ion channels might locate in the two types of receptors. However, the relevant mechano-electrical transductive molecule remains to be clarified.

Key words: baroreceptor, benzamil, ruthenium red, mechanosensitive channel, epithelial sodium channel, acidsensitive channel

中图分类号:

R 331.3⁺6

刘廷会;魏华;赵海燕;刘萍;黄海霞;王伟;付小锁;钮伟真. 苄阿米洛利和钌红对颈动脉窦压力感受器放电的阻断作用[J]. 首都医科大学学报, 2011, 32(2): 218-223.

LIU Ting-hui;WEI Hua;ZHAO Hai-yan;LIU Ping;HUANG Hai-xia;WANG Wei;FU Xiao-suo;NIU Wei-zhen. Blocking Effects of Benzamil and Ruthenium Red onCarotid Baroreceptor Discharges[J]. Journal of Capital Medical University, 2011, 32(2): 218-223.

[1]	赵海燕, 黄海霞, 刘萍, 张思韬, 钮伟真, 王伟. 不同动脉压力感受器对钌红反应的敏感性差异[J]. 首都医科大学学报, 2014, 35(5): 640-645.
[2]	赵海燕;魏华;刘萍;刘廷会;王伟;黄海霞;钮伟真. 窦神经压力感受器聚类分析[J]. 首都医科大学学报, 2009, 30(5): 658-663.