不同动脉压力感受器对钌红反应的敏感性差异

doi:10.3969/j.issn.1006-7795.2014.05.024

首都医科大学学报 ›› 2014, Vol. 35 ›› Issue (5): 640-645.doi: 10.3969/j.issn.1006-7795.2014.05.024

不同动脉压力感受器对钌红反应的敏感性差异

赵海燕¹, 黄海霞², 刘萍², 张思韬², 钮伟真², 王伟²

1. 首都医科大学燕京医学院机能教研室, 北京 100069;
2. 首都医科大学基础医学院生理学与病理生理学系, 北京 100069

收稿日期:2014-03-05 出版日期:2014-10-21 发布日期:2014-10-20
通讯作者: 王伟 E-mail:wangwei@ccmu.edu.cn
基金资助:
北京市教育委员会科技成果转化与产业化项目（PXM2007-014226-050086）。

Distinct sensitivity of different arterial baroreceptors on ruthenium red

Zhao Haiyan¹, Huang Haixia², Liu Ping², Zhang Sitao², Niu Weizhen², Wang Wei²

1. Department of Medical Functional Science, Yanjing Medical College, Capital Medical University, Beijing 100069, China;
2. Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2014-03-05 Online:2014-10-21 Published:2014-10-20
Supported by:
This study was supported by Beijing Municipal Commission of Education (PXM2007-014226-050086).

摘要/Abstract

摘要：

目的研究钌红对压力感受器不同纤维的作用是否存在差异以及这些差异与感受器纤维类型的关系。方法制备家兔离体颈动脉窦-窦神经标本，采用颈动脉窦窦内外双重灌流，钳制窦内压同时记录窦神经细束（含少量纤维）放电，然后应用Spike2软件分析，获得单纤维放电的信息。结果在20 μmol/L钌红作用下，根据对钌红的反应，可将窦神经压力感受纤维分为钌红敏感纤维和钌红不敏感纤维2类。进一步分析发现这2类纤维的电生理特点分别与C类和A类压力感受器相对应。结论窦神经中存在对钌红反应不同的2类纤维，可能分属A类纤维和C类纤维，提示这2类压力感受器的机-电换能机制可能存在某些差异。

关键词: 动脉压力感受器, 钌红, 钌红敏感纤维

Abstract:

Objective To investigate if ruthenium red (RR) possesses differentiating effects on baroreceptors and to understand the relationship between these different effects and types of the baroreceptors. Methods Carotid sinus-nerve preparation of the rabbit was perfused intrasinusally and extrasinusally in vitro. Baroreceptor discharges were recorded from nerve filaments containing 2-5 fibers while the intrasinusal pressure was clamped with pulsatile or ramp waves. Single fiber discharges were obtained by sorting the few-fiber discharge using software Spike 2. Results According to the response to RR (20 μmol /L), baroreceptor fibers can be distinguished into two types: RR sensitive and insensitive fibers. To further analyze electrophysiological characteristics of the two types, we found that the RR sensitive and insensitive fibers correspond with C- and A-fibers, respectively.Conclusion Carotid sinus fibers can be classified into two types according to the response to RR (20 μmol /L). The two types of baroreceptors might belong to A- and C-fibers, respectively. The results suggested that the mechanisms for mechano-electrical transduction in the two types of baroreceptor fibers might not be the same.

Key words: baroreceptor, ruthenium red, ruthenium red sensitive fibers

中图分类号:

R329.3

赵海燕, 黄海霞, 刘萍, 张思韬, 钮伟真, 王伟. 不同动脉压力感受器对钌红反应的敏感性差异[J]. 首都医科大学学报, 2014, 35(5): 640-645.

Zhao Haiyan, Huang Haixia, Liu Ping, Zhang Sitao, Niu Weizhen, Wang Wei. Distinct sensitivity of different arterial baroreceptors on ruthenium red[J]. Journal of Capital Medical University, 2014, 35(5): 640-645.

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不同动脉压力感受器对钌红反应的敏感性差异

Distinct sensitivity of different arterial baroreceptors on ruthenium red

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