多巴胺受体在大鼠胃体肌间神经丛氮能神经元的定位

doi:10.3969/j.issn.1006-7795.2015.03.017

首都医科大学学报 ›› 2015, Vol. 36 ›› Issue (3): 426-431.doi: 10.3969/j.issn.1006-7795.2015.03.017

多巴胺受体在大鼠胃体肌间神经丛氮能神经元的定位

樊瑞芳, 郑丽飞, 冯小燕, 张晓丽, 张悦, 朱进霞

首都医科大学基础医学院生理与病理生理学系, 北京 100069

收稿日期:2015-02-26 出版日期:2015-06-21 发布日期:2015-06-15
通讯作者: 朱进霞 E-mail:zhu_jx@ccmu.edu.cn
基金资助:
国家自然科学基金(81370482, 31400991),北京市自然科学基金 (7132017)项目, 北京市属高等学校创新团队建设与教师职业发展计划项目 (IDHT20140514).

The localization of dopamine receptors on the nitrergic neurons in gastric myenteric plexus of rats

Fan Ruifang, Zheng Lifei, Feng Xiaoyan, Zhang Xiaoli, Zhang Yue, Zhu Jinxia

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2015-02-26 Online:2015-06-21 Published:2015-06-15
Supported by:
This work was supported by the National Natural Science Foundation of China (81370482 and 31400991), Natural Science Foundation of Beijing (7132017), the Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality (IDHT20140514).

摘要/Abstract

摘要：

目的研究5种多巴胺受体在大鼠胃体肌间神经丛一氧化氮能神经元的分布.方法本文主要采用冰冻切片和全层铺片的免疫荧光组织化学方法观察胃体肌间神经丛中多巴胺受体的分布,以及与一氧化氮能神经元的共定位情况.结果 5种多巴胺受体亚型的免疫荧光和一氧化氮合酶的免疫荧光在胃体肌间神经丛均有分布,而且在一氧化氮合酶免疫阳性的神经元上存在有多巴胺受体D₁、D₂、D₃ 和D₅的免疫阳性,而D₄没有明显的免疫阳性表达.结论多巴胺受体D₁、D₂、D₃ 和D₅分布在胃体肌间神经丛一氧化氮能神经元上,可能通过调节一氧化氮的合成和释放进而调节胃体平滑肌的运动.

关键词: 多巴胺受体, 胃肌间神经丛, 一氧化氮能神经元

Abstract:

Objective To investigate the distribution of dopamine receptors in neural nitric oxide synthase (nNOS)immunoreactive neurons in myenteric plexus of gastric corpus. Methods Immunofluorescence for frozen sections of gastric corpus and whole mount stretched preparation of myenteric plexus were used to investigate the colocolization of dopamine receptors and nNOS in myenteric neurons. Results The immunoreactivities of five subtypes' dopamine receptors and nNOS were largely distributed in the enteric neurons of gastric myenteric plexus. Furthermore, the Immunofluorescence of dopamine receptor D₁, D₂, D₃ and D₅, but not D₄ were observed in the nNOS-immunoreactive neurons. Conclusion Dopamine receptor D₁, D₂, D₃ and D₅ were distributed in the gastric nitrergic neurons of gastric corpus, which might be involved in the NO synthesis, and then regulation of gastric motility.

Key words: dopamine receptors, gastric myenteric plexus, nitrergic neuron

中图分类号:

R333

樊瑞芳, 郑丽飞, 冯小燕, 张晓丽, 张悦, 朱进霞. 多巴胺受体在大鼠胃体肌间神经丛氮能神经元的定位[J]. 首都医科大学学报, 2015, 36(3): 426-431.

Fan Ruifang, Zheng Lifei, Feng Xiaoyan, Zhang Xiaoli, Zhang Yue, Zhu Jinxia. The localization of dopamine receptors on the nitrergic neurons in gastric myenteric plexus of rats[J]. Journal of Capital Medical University, 2015, 36(3): 426-431.

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多巴胺受体在大鼠胃体肌间神经丛氮能神经元的定位

The localization of dopamine receptors on the nitrergic neurons in gastric myenteric plexus of rats

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