多巴胺受体在人与小鼠胃黏膜泌生长抑素细胞中的分布

doi:10.3969/j.issn.1006-7795.2015.03.018

首都医科大学学报 ›› 2015, Vol. 36 ›› Issue (3): 432-436.doi: 10.3969/j.issn.1006-7795.2015.03.018

多巴胺受体在人与小鼠胃黏膜泌生长抑素细胞中的分布

孙路, 冯小燕, 郑丽飞, 张晓丽, 张悦, 朱进霞

首都医科大学基础医学院生理与病理生理学系, 北京 100069

收稿日期:2015-03-26 出版日期:2015-06-21 发布日期:2015-06-15
通讯作者: 朱进霞 E-mail:zhu_jx@ccmu.edu.cn
基金资助:
国家自然科学基金(81370482, 31400991),北京市自然科学基金 (7132017)项目, 北京市属高等学校创新团队建设与教师职业发展计划项目 (IDHT20140514).

Distribution of dopamine receptors in gastric mucosal somatostatin-secreting cell of human and mice

Sun Lu, Feng Xiaoyan, Zheng Lifei, Zhang Xiaoli, Zhang Yue, Zhu Jinxia

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2015-03-26 Online:2015-06-21 Published:2015-06-15
Supported by:
This work was supported by the National Natural Science Foundation of China (81370482 and 31400991), Natural Science Foundation of Beijing (7132017), the Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges under Beijing Municipality (IDHT20140514).

摘要/Abstract

摘要：

目的研究人与小鼠的胃黏膜泌生长抑素细胞(D细胞)上多巴胺D₁、D₂受体的分布.方法采用冰冻切片免疫荧光染色的方法分别对多巴胺受体在人与小鼠胃体D细胞的分布进行检测.结果多巴胺D₁、D₂受体均分布在人与小鼠的胃体黏膜层,且D细胞上存在有D₂受体的免疫阳性表达,而未见D₁受体的阳性表达,人与小鼠结果保持一致.结论多巴胺D₂受体分布于D细胞上,可能介导多巴胺对生长抑素分泌的调节进而参与调节胃的分泌及运动功能.

关键词: 生长抑素, 多巴胺受体, D细胞

Abstract:

Objective To investigate the distribution of dopamine D₁, D₂ receptors in somatostatin-secreting D cells in gastric corpus mucosa in human and mice. Methods Immunofluorescence was used to detect the expression of dopamine receptors on the somatostatin-secreting D cells in human or mice gastric corpus. Results Abundant immunofluorescence reactivities (IR) of dopamine D₁ and D₂ receptors, and somatostatin-IR cells were distributed in the gastric corpus mucosa. Furthermore, D₂ receptors-IR, not D₁ receptors-IR, was observed in the somatostatin-IR cells of mice and human gastric corpus mucosa. Conclusion Dopamine D₂ receptors, not D₁ receptors were distributed in the gastric somatostatin-secreting D cells of both human and mice, which might mediate the regulation of dopamine on the somatostatin secretion, gastric secretion and motility.

Key words: somatostatin, dopamine receptors, gastric D cell

中图分类号:

R333

孙路, 冯小燕, 郑丽飞, 张晓丽, 张悦, 朱进霞. 多巴胺受体在人与小鼠胃黏膜泌生长抑素细胞中的分布[J]. 首都医科大学学报, 2015, 36(3): 432-436.

Sun Lu, Feng Xiaoyan, Zheng Lifei, Zhang Xiaoli, Zhang Yue, Zhu Jinxia. Distribution of dopamine receptors in gastric mucosal somatostatin-secreting cell of human and mice[J]. Journal of Capital Medical University, 2015, 36(3): 432-436.

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多巴胺受体在人与小鼠胃黏膜泌生长抑素细胞中的分布

Distribution of dopamine receptors in gastric mucosal somatostatin-secreting cell of human and mice

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