Snail与Claudin-3蛋白的表达与原发性肝癌发生及预后的关联性研究

doi:10.3969/j.issn.1006-7795.2018.03.015

首都医科大学学报 ›› 2018, Vol. 39 ›› Issue (3): 393-397.doi: 10.3969/j.issn.1006-7795.2018.03.015

Snail与Claudin-3蛋白的表达与原发性肝癌发生及预后的关联性研究

申燕军¹, 陈京龙², 车娟娟¹, 王婧¹, 曹邦伟¹

1. 首都医科大学附属北京友谊医院肿瘤中心, 北京 100050;
2. 首都医科大学附属北京地坛医院肿瘤科, 北京 100015

收稿日期:2017-05-27 出版日期:2018-05-21 发布日期:2018-06-11
通讯作者: 曹邦伟 E-mail:oncology@ccmu.edu.cn
基金资助:
北京市自然科学基金（7172081）。

Occurrence and prognosis value of Snail and Claudin-3 expression in hepatocelluar carcinoma

Shen Yanjun¹, Chen Jinglong², Che Juanjuan¹, Wang Jing¹, Cao Bangwei¹

1. Cancer Center, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China;
2. Department of Oncology, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China

Received:2017-05-27 Online:2018-05-21 Published:2018-06-11
Supported by:
This study was supported by Natural Science Foundation of Beijing (7172081).

摘要/Abstract

摘要： 目的本研究通过收集原发性肝细胞癌（hepatocellular carcinoma，HCC）患者的临床资料，并检测癌及癌旁组织中Claudin-3和Snail蛋白的表达，探讨Claudin-3和Snail蛋白与肝癌患者临床病理特征与预后的关联。方法收集根治性切除的肝细胞癌病例患者资料，利用免疫组织化学的方法检测82例HCC组织及60例癌旁组织中Snail、Claudin-3蛋白的表达。采用Kaplan-Meier法进行生存分析，应用Cox比例风险模型进行多因素回归分析。结果肝癌组织中Snail蛋白阳性表达69.47%，高于癌旁组织，差异有统计学意义（P=0.001）；Claudin-3阳性表达47.61%，低于癌旁组织，差异有统计学意义（P=0.002）；两者的表达呈负相关（r=-0.430，P<0.01）；Snail蛋白在HCC中阳性表达明显高于癌旁组织，在病理分期、微血管侵犯相关方面差异有统计学意义（P<0.05）；在Cox多因素回归分析中，Snail、Claudin-3的表达是HCC患者生存的独立危险因素（P<0.05）。结论 Snail与Claudin-3蛋白与HCC的发生、侵袭有一定的关联性。进行HCC患者Snail、Claudin-3蛋白的联合检测，在临床实践中对HCC患者病理学特征与预后的判断具有重要的提示作用。

关键词: 肝细胞肝癌, Snail, Claudin-3

Abstract: Objective This study aimed to investigate the relationship between the expression of Claudin-3 or Snail and the clinical pathology and prognosis in the hepatocellular carcinoma (HCC) patients by detecting the expression of Claudin-3 and Snail in the HCC and liver cancer-adjacent tissues.Methods Snail and Claudin-3 were detected in cases of 60 liver cancer-adjacent tissues and 82 cases of HCC by using immunohistochemical technique. The postoperative prognostic factors were assessed by univariate Kaplan-Meier analysis and a multivariate Cox proportional hazard model.Results Snail positive expression was 69.47% in HCC and was significantly higher than liver cancer-adjacent tissues (P=0.001). Claudin-3 proteins positive expression were 47.61%in HCC and were significantly lower than liver cancer-adjacent tissue (P=0.002). The expression of Snail and Claudin-3 was negatively correlated (r=-0.430, P<0.01). Snail positive expression in HCC was significantly higher than liver cancer-adjacent tissues, and the difference was statistically significant in microvascular invasion and stages of pathology tumor lymph node metastasis (P<0.05). Multivariate analysis by using Cox regression model identified that Snail and Claudin-3 were independent prognostic factors of HCC (P<0.05).Conclusion The correlation between the Snail or Claudin-3 and the occurrence and invasion in HCC were exhibited. The combined detection of Snail and Claudin-3 protein in HCC patients might be an important pathological and prognosis biomarker in HCC patients.

Key words: hepatocellular carcinoma, Snail, Claudin-3

中图分类号:

R730.5

申燕军, 陈京龙, 车娟娟, 王婧, 曹邦伟. Snail与Claudin-3蛋白的表达与原发性肝癌发生及预后的关联性研究[J]. 首都医科大学学报, 2018, 39(3): 393-397.

Shen Yanjun, Chen Jinglong, Che Juanjuan, Wang Jing, Cao Bangwei. Occurrence and prognosis value of Snail and Claudin-3 expression in hepatocelluar carcinoma[J]. Journal of Capital Medical University, 2018, 39(3): 393-397.

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Snail与Claudin-3蛋白的表达与原发性肝癌发生及预后的关联性研究

Occurrence and prognosis value of Snail and Claudin-3 expression in hepatocelluar carcinoma

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