超声二维斑点追踪技术分析小鼠颈动脉僵硬度的方法学研究

doi:10.3969/j.issn.1006-7795.2018.06.016

首都医科大学学报 ›› 2018, Vol. 39 ›› Issue (6): 883-888.doi: 10.3969/j.issn.1006-7795.2018.06.016

超声二维斑点追踪技术分析小鼠颈动脉僵硬度的方法学研究

许晴¹, 刘丽娜¹, 宋妍婷², 曲爱娟²

1. 首都医科大学中心实验室, 北京 100069;
2. 首都医科大学基础医学院生理学与病理生理学系重塑相关心血管疾病教育部重点实验室代谢紊乱相关心血管疾病北京市重点实验室, 北京 100069

收稿日期:2018-10-09 出版日期:2018-11-21 发布日期:2018-12-19
通讯作者: 曲爱娟 E-mail:aijuanqu@ccmu.edu.cn
基金资助:
国家自然科学基金面上项目（81701099），首都医科大学校长基金（2016JS20）。

Assessing stiffness of carotid artery via two-dimensional speckle-tracking in mice

Xu Qing¹, Liu Lina¹, Song Yanting², Qu Aijuan²

1. Core Facilities Centre, Capital Medical University, Beijing 100069, China;
2. Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University;Key Laboratory of Remodeling-related Cardiovascular Diseases, Ministry of Education;Beijing Key Laboratory of Metabolic Disorder-related Cardiovascular Diseases, Beijing 100069, China

Received:2018-10-09 Online:2018-11-21 Published:2018-12-19
Supported by:
This study was supported by National Natural Science Foundation of China(81701099), President's Fund of Capital Medical University(2016JS20).

摘要/Abstract

摘要： 目的探讨超高分辨率小动物超声影像系统二维斑点追踪技术分析小鼠颈动脉血管壁僵硬度的方法。方法选用6只正常8周龄C57B1 /6J雄性小鼠及6只60周龄载脂蛋白E（apolipoprotein E）基因敲除小鼠（ApoE^－/－），应用超高分辨率小动物专用超声影像系统及50 MHz探头，测定小鼠颈动脉内膜及中膜厚度（intima-media thickness，IMT）、血管平均面积、血管平均直径及面积变化率和直径变化率、血管扩张性等指标，同时测量血管壁整体径向应变及脉搏波传导速度（pulse wave velocity，PWV），以此评价小鼠颈动脉血管壁僵硬度。结果与正常小鼠相比，ApoE^－/－小鼠IMT增加［（0.05±0.01）mm vs（0.02±0.01）mm］，面积变化率［（49.64±5.69）% vs（34.33±5.92）%］和直径变化率［（25.21±3.04）% vs（17.33±3.14%）］下降；血管扩张性降低［（100.49±12.40）MPa^－1 vs（59.87±21.22）MPa^－1］，脉搏波传导速度增快［（1.90±0.37）m/s vs（3.09±0.23）m/s］；血管壁整体径向应变降低［（66.97±10.28）% vs（41.64±8.51）%］，差异有统计学意义（n=6， P<0.05）。结论应用二维斑点追踪技术分析小鼠颈动脉血管壁运动僵硬度的方法是可行的，可为小动物血管僵硬度检测提供新的方法。

关键词: 超高频超声, 二维斑点追踪技术, 血管僵硬度, 无创检测, 小鼠

Abstract: Objective To explore a novel non-invasive method for the assessment of carotid artery stiffness by advanced speckle tracking algorithms by high-resolution ultrasound in mice. Methods Six 8-week-old C57B1/6J male mice and six 60-week-old Apolipoprotein E knockout mice (ApoE^-/-) were subjected to t ultrasound scan imaging through the Vevo 2100 with the MS-700 at a center frequency of 50MHz. Carotid intima-media thickness (IMT), mean diameter, mean area, area percent spread, diameter percent spread, distensibility, pulse wave velocity and global radial strain were quantified to evaluate the stiffness of the carotid artery wall in mice. Results Compared with C57 mice, ApoE^-/- mice had increased IMT[(0.05±0.01)mm vs (0.02±0.01)mm] but decreased area percent spread[(49.64±5.69)% vs (34.33±5.92)%], diameter percent spread[(25.21±3.04)% vs (17.33±3.14)%] and distensibility[(100.49±12.40)MPa^-1 vs (59.87±21.22)MPa^-1]. The pulse wave velocity was increased[(1.90±0.37)m/s vs (3.09±0.23)m/s], the global radial strain was reduced[(66.97±10.28)% vs (41.64±8.51)%] in ApoE^-/- mice. The difference was statistically significant (n=6, P<0.05). Conclusion Our study suggested that the two-dimensional speckle tracking technology was feasible for evaluating the stiffness of carotid artery wall in mice, thus providing a novel non-invasive method for the vessel stiffness assessment in rodents.

Key words: high-resolution ultrasonography, two-dimensional speckle-tracking, stiffness, noninvasive detection, mice

中图分类号:

R543.4

许晴, 刘丽娜, 宋妍婷, 曲爱娟. 超声二维斑点追踪技术分析小鼠颈动脉僵硬度的方法学研究[J]. 首都医科大学学报, 2018, 39(6): 883-888.

Xu Qing, Liu Lina, Song Yanting, Qu Aijuan. Assessing stiffness of carotid artery via two-dimensional speckle-tracking in mice[J]. Journal of Capital Medical University, 2018, 39(6): 883-888.

参考文献

[1] Vlachopoulos C, Aznaouridis K, Stefanadis C. Prediction of cardiovascular events and all-cause mortality with arterial stiffness:a systematic review and meta-analysis[J]. J Am Coll Cardiol, 2010, 55(13):1318-1327.
[2] Mozaffarian D, Benjamin E J, Go A S, et al.Heart disease and stroke statistics-2015 update:a report from the American Heart Association[J]. Circulation, 2015,131(4):e29-e322.
[3] Lovic D, Kallistratos M S, Poulimenos L, et al. Pulse wave velocity:retrospective analysis in a balkan normotensive and hypertensive population:a study of 9923 patients[J]. Angiology, 2018, 69(1):59-64.
[4] Brand A, Bathe M, Oertelt-Prigione S, et al. Right heart function in impaired left ventricular diastolic function:2D speckle tracking echocardiography-based and doppler tissue imaging-based analysis of right atrial and ventricular function[J]. Echocardiography, 2018, 35(1):47-55.
[5] Cavalcante J L, Lima J A, Redheuil A, et al. Aortic stiffness:current understanding and future directions[J]. J Am Coll Cardiol, 2011, 57(14):1511-1522.
[6] 李爽, 王薇, 洪慧. 视网膜分支静脉阻塞与颈内动脉弹性功能参数相关性研究[J]. 首都医科大学学报, 2017, 38(1):6-10.
[7] Di Lascio N, Kusmic C, Stea F, et al. Longitudinal micro-ultrasound assessment of the ob/ob mouse model:evaluation of cardiovascular, renal and hepatic parameters[J]. Int J Obes (Lond), 2018,42(3):518-524.
[8] Sabia L, Avenatti E, Cesareo M, et al. Evaluation of aortic stiffness by a new simplified 2D speckle tracking analysis[J]. Int J Cardiovasc Imaging, 2018,34:1753-1760.
[9] Liu H, Li X, Song Y, et al. MicroRNA-217 attenuates intima-media complex thickness of ascending aorta measured by ultrasound bio-microscopy and inhibits inflammation and lipid metabolism in atherosclerotic models of ApoE^-/- mice[J]. Lipids Health Dis,2018, 17(1):170.
[10] Oishi Y, Mizuguchi Y, Miyoshi H, et al. A novel approach to assess aortic stiffness related to changes in aging using a two-dimensional strain imaging[J]. Echocardiography, 2008, 25(9):941-945.
[11] Nasiri-Ansari Ν, Dimitriadis G K, Agrogiannis G, et al. Canagliflozin attenuates the progression of atherosclerosis and inflammation process in APOE knockout mice[J]. Cardiovasc Diabetol,2018, 17(1):106.
[12] Ding S, Jiang J, Yu P, et al. Green tea polyphenol treatment attenuates atherosclerosis in high-fat diet-fed apolipoprotein E-knockout mice via alleviating dyslipidemia and up-regulating autophagy[J]. PLoS One, 2017, 12(8):e0181666.
[13] Cohn J N. Arterial stiffness,vascular disease, and risk of cardiovascular events[J].Circulation,2006,113(5):601-603.
[14] Sumbul H E, Koc A S, Demirtas D. Increased carotid-femoral pulse wave velocity and common carotid artery intima-media thickness obtained to assess target organ damage in hypertensive patients are closely related[J]. Clin Exp Hypertens, 2018, Aug 24:1-8. doi:10.1080/10641963.2018.1506471.[Epub ahead of print].
[15] 王君玓, 商广芸, 何为. 2型糖尿病病人大动脉僵硬度相关危险因素研究[J]. 首都医科大学学报,2017,38(5):740-743.
[16] 谢晓亮, 李觉, 胡大一. 脉搏波传导速度测定方法及临床意义[J]. 中国心血管病研究杂志, 2007, 5(6):465-468.
[17] 仲恒,徐丽梅.脉搏波传导速度的临床应用研究进展[J].解放军医药杂志,2015,27(9):53-56.
[18] Bieseviciene M, Vaskelyte J J, Mizariene V, et al.Two-dimensional speckle-tracking echocardiography for evaluation of dilative ascending aorta biomechanics[J]. BMC Cardiovasc Disord, 2017, 17(1):27.
[19] Bu Z, Ma J, Fan Y, et al. Ascending aortic strain analysis using 2-Dimensional speckle tracking echocardiography improves the diagnostics for coronary artery stenosis in patients with suspected stable angina pectoris[J]. J Am Heart Assoc, 2018, 7(14).
[20] Demetgul H, Giray D, Delibas A, et al. 2D-Speckle tracking echocardiography contributes to early identification of impaired left ventricular myocardial function in children with chronic kidney disease[J]. Cardiol Young, 2018, 28:1-6.
[21] Salas-Pacheco J L, Ávila-Vanzzini N, Eugenia R M, et al. Left atrium function by 2D speckle tracking in aortic valve disease[J]. Echocardiography, 2016, 33(12):1828-1834.

超声二维斑点追踪技术分析小鼠颈动脉僵硬度的方法学研究

Assessing stiffness of carotid artery via two-dimensional speckle-tracking in mice

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