pH值和剂量对腹腔注射L-精氨酸诱导小鼠急性胰腺炎模型的影响

doi:10.3969/j.issn.1006-7795.2017.05.014

首都医科大学学报 ›› 2017, Vol. 38 ›› Issue (5): 700-703.doi: 10.3969/j.issn.1006-7795.2017.05.014

pH值和剂量对腹腔注射L-精氨酸诱导小鼠急性胰腺炎模型的影响

肖鲁瑶¹, 张杰¹, 郑帅², 梅少帅³

1. 首都医科大学附属北京安贞医院消化内科, 北京 100029;
2. 首都医科大学附属北京安贞医院心肺血管疾病研究所, 北京 100029;
3. 首都医科大学附属北京安贞医院病理科, 北京 100029

收稿日期:2017-06-20 出版日期:2017-09-21 发布日期:2017-10-18
通讯作者: 张杰 E-mail:zhangjie4155@sina.com
基金资助:
国家自然科学基金（81470889）。

Effects of pH values and doses on intraperitoneal injection of L-arginine induced acute pancreatitis in mice

Xiao Luyao¹, Zhang Jie¹, Zheng Shuai², Mei Shaoshuai³

1. Department of Gastroenterology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China;
2. Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China;
3. Department of Pathology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China

Received:2017-06-20 Online:2017-09-21 Published:2017-10-18
Supported by:
This study was supported by National Natural Science Foundation of China(81470889).

摘要/Abstract

摘要： 目的探讨L-精氨酸（L-arginine）不同给药方法诱导小鼠急性胰腺炎模型的效果。方法 L-精氨酸溶液以不同pH值、不同剂量诱导小鼠急性胰腺炎模型，造模后2、4、12、24 h统计小鼠病死率，采集小鼠血浆以淀粉-碘比色法检测淀粉酶活性，取胰腺及肺组织行石蜡切片HE染色观察组织损伤严重程度。结果 pH值为11高剂量组小鼠2 h病死率100%，其余各组各时间点小鼠均存活。3个模型组与对照组相比血浆淀粉酶活性、胰腺组织病理评分均升高，差异有统计学意义（P<0.05）。模型组之间比较，pH值为7高剂量组胰腺及肺组织损伤最重。结论 L-精氨酸溶液的pH值及给药剂量对胰腺损伤程度有不同程度的影响，20% L-精氨酸溶液pH值为7按4 g/kg的剂量间隔1 h连续2次腹腔注射诱导的小鼠急性胰腺炎模型组织损伤重，且小鼠存活率高。

关键词: 小鼠, L-精氨酸, 急性胰腺炎, 动物模型

Abstract: Objective To investigate the effects of different methods of L-arginine injection on acute pancreatitis model mice. Methods Mice suffering from acute pancreatitis were induced by different pH values and different doses of L-arginine, then mortality was calculated, and plasma amylase activity was detected by starch-iodine colorimetry, and pancreas and lung tissue injury severity was observed by paraffin section of HE staining at the time of 2, 4, 12, 24 h after last injection. Results Mortality in pH11 high-dose group was 100% at 2 h, and mice in other groups all survived. Compared with the control group, the plasma amylase activity and pancreas pathological score were significantly increased in all model groups (P<0.001).Compared with the other model groups, pancreas and lung tissue injury was the most severe in pH7 high-dose group. Conclusion The pH value and dosage of L-arginine solution have significant impacts on the degree of pancreatic injury. Acute pancreatitis mice model induced by 2 i.p. injections of L-arginine (400 mg/kg body weight) in 0.9% sodium chloride (pH7) at 1-hour intervals was the most severe compared with the other model groups, and with high survival rate.

Key words: mice, L-arginine, acute pancreatitis, animal model

中图分类号:

R576

肖鲁瑶, 张杰, 郑帅, 梅少帅. pH值和剂量对腹腔注射L-精氨酸诱导小鼠急性胰腺炎模型的影响[J]. 首都医科大学学报, 2017, 38(5): 700-703.

Xiao Luyao, Zhang Jie, Zheng Shuai, Mei Shaoshuai. Effects of pH values and doses on intraperitoneal injection of L-arginine induced acute pancreatitis in mice[J]. Journal of Capital Medical University, 2017, 38(5): 700-703.

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pH值和剂量对腹腔注射L-精氨酸诱导小鼠急性胰腺炎模型的影响

Effects of pH values and doses on intraperitoneal injection of L-arginine induced acute pancreatitis in mice

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