同步辐射X线相衬成像对裸鼠胰腺癌的早期诊断

doi:10.3969/j.issn.1006-7795.2019.02.008

首都医科大学学报 ›› 2019, Vol. 40 ›› Issue (2): 195-200.doi: 10.3969/j.issn.1006-7795.2019.02.008

• 肿瘤的免疫治疗与代谢 • 上一篇下一篇

同步辐射X线相衬成像对裸鼠胰腺癌的早期诊断

王佳¹, 乐凯¹, 郭懿樊¹, 郭旭¹, 张涛¹, 任宇¹, 王睿斌¹, 许光中¹, 郑海亮², 冯树理², 朱斌¹

1. 首都医科大学附属北京世纪坛医院普通外科及肿瘤外科, 北京 100038;
2. 首都医科大学附属北京世纪坛医院医学工程处, 北京 100038

收稿日期:2019-01-17 出版日期:2019-03-21 发布日期:2019-04-15
通讯作者: 朱斌 E-mail:binbinzhu99@hotmail.com
基金资助:
北京市自然科学基金面上项目（1152003），北京市教育委员会科技计划一般项目（KM201710025025）。

Early diagnosis of pancreatic cancer in a nude mice model on synchrotron radiation X-ray phase contrast imaging

Wang Jia¹, Le Kai¹, Guo Yifan¹, Guo Xu¹, Zhang Tao¹, Ren Yu¹, Wang Ruibin¹, Xu Guangzhong¹, Zheng Hailiang², Feng Shuli², Zhu Bin¹

1. Department of General Surgery & Surgical Oncology, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China;
2. Department of Medical Engineering, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China

Received:2019-01-17 Online:2019-03-21 Published:2019-04-15
Supported by:
This study was supported by Natural Science Foundation of Beijing (1152003), General Items of Science and Technology Project of Beijing Municipal Education Commission (KM201710025025).

摘要/Abstract

摘要： 目的通过建立裸鼠胰腺癌模型，结合磁共振成像（magnetic resonance imaging，MRI），探讨同步辐射X线相衬成像技术对胰腺癌的早期诊断。方法 32只nu/nu雌鼠，采用数字表法随机分为A、B两组：A组（n=24）为人胰腺癌细胞PANC-1胰腺原位移植瘤组，即于胰腺被膜下注射10⁸×2 μL单细胞悬浮液；B组（n=8）为空白对照组，即于胰腺被膜下注射2 μL 0.9%（质量浓度）氯化钠注射液。再根据术后1、2、3、4周处理时间的不同，将A、B两组分为4个亚组，即A1、A2、A3、A4（n=6）和B1、B2、B3、B4（n=2）。术后分别行MRI扫描、肿瘤标本相衬成像及病理学检查。结果 ①A组术后平均成瘤率73%（16/22），术后4周成瘤率最高，达到100%，成瘤率随观察时间的延长而升高，二者之间存在线性趋势关系（χ_线性趋势²=4.718，P=0.030）；病理组织学检查为胰腺低分化腺癌。②同步辐射X线相衬成像可显示直径为0.5 mm的胰腺原位肿瘤，早期（两周）的灵敏度和特异度达到100%，明显高于MRI扫描（P=0.029）。结论 PANC-1细胞原位移植是建立裸鼠胰腺癌模型的可靠方法；与MRI相比，同步辐射X线相衬成像能发现胰腺癌微小病变，有可能为胰腺癌的早期诊断提供了一种全新的技术手段。

关键词: 胰腺癌, 动物模型, 原位移植, 同步辐射X线相衬成像, 磁共振成像

Abstract: Objective To explore the diagnostic value of synchrotron radiation X-ray phase contrast imaging compared with magnetic resonance imaging (MRI) in early diagnosis of pancreatic cancer with an orthotopic xenograft nude mice model of pancreatic cancer. Methods Thirty-two female nude mice were divided into 2 groups of 4 subgroups randomly, subgroups were labeled A1, A2, A3, A4 (n=6) and B1, B2, B3, B4 (n=2) as the different intervals at 1, 2, 3, and 4 weeks postoperatively, respectively. 10⁸ cells/mL×20μL PANC-1 cell suspension was injected into pancreatic capsula of group A but 20μL sterile saline was injected in the group B. MRI scans were performed at 1, 2, 3, and 4 weeks postoperatively and the specimens were excised from nude mice and fixed in 10% formalin immediately. Synchrotron radiation X-ray phase contrast imaging was performed followed by histopathological examination. Results ①Low grade adenocarcinoma was confirmed with pathology, and the average rate of tumorigenesis in group A was 73% (16/22), and the rate was the highest after 4 weeks, which was 100%. With the time passing, the rate of tumorigenesis increased with the linear trend test (χ²=4.718, P=0.030). ②The image of tumor with a diameter of 0.5 mm in pancreas was observed with synchrotron radiation phase contrast imaging. The sensitivity and specificity of the early (two weeks) reached 100%, significantly higher than that of magnetic resonance imaging (P=0.029). Conclusion PANC-1 cells injected into pancreatic capsula was a reliable method for establishment of pancreatic cancer model. Compared with MRI, synchrotron radiation contrast imaging could detect tiny lesions of pancreatic cancer, which may provide a new method for early diagnosis of pancreatic cancer.

Key words: pancreatic cancer, animal model, orthotopic transplantation, synchrotron radiation X-ray phase contrast imaging, magnetic resonance imaging

中图分类号:

R735.9

王佳, 乐凯, 郭懿樊, 郭旭, 张涛, 任宇, 王睿斌, 许光中, 郑海亮, 冯树理, 朱斌. 同步辐射X线相衬成像对裸鼠胰腺癌的早期诊断[J]. 首都医科大学学报, 2019, 40(2): 195-200.

Wang Jia, Le Kai, Guo Yifan, Guo Xu, Zhang Tao, Ren Yu, Wang Ruibin, Xu Guangzhong, Zheng Hailiang, Feng Shuli, Zhu Bin. Early diagnosis of pancreatic cancer in a nude mice model on synchrotron radiation X-ray phase contrast imaging[J]. Journal of Capital Medical University, 2019, 40(2): 195-200.

参考文献

[1] 张师容, 靳伟, 刘亮, 等. 2017年胰腺癌基础研究及诊疗新进展[J]. 中国癌症杂志, 2018, 28(1):1-10.
[2] Desantis C E, Siegel R L, Sauer A G, et al. Cancer statistics for african Americans, 2016:Progress and opportunities in reducing racial disparities[J]. CA Cancer J Clin, 2016, 66(4):290-308.
[3] Chen W, Zheng R, Zhang S, et al. Cancer incidence and mortality in China in 2013:an analysis based on urbanization level[J]. Chin J Cancer Res, 2017, 29(1):1-10.
[4] Zhang L, Sanagapalli S, Stoita A. Challenges in diagnosis of pancreatic cancer[J]. World J Gastroenterol, 2018, 24(19):2047-2060.
[5] Halbrook C J, Lyssiotis C A. Employing metabolism to improve the diagnosis and treatment of pancreatic cancer[J]. Cancer Cell, 2017, 31(1):5-19.
[6] 王红艳, 马云川, 李德鹏, 等. 18F-FDGPET显像在胰腺癌诊断中的应用[J]. 首都医科大学学报, 2008, 29(1):42-44.
[7] Chen Y, Gao S, Chen J, et al. Serum CA242, CA199, CA125, CEA, and TSGF are biomarkers for the efficacy and prognosis of cryoablation in pancreatic cancer patients[J]. Cell Biochem Biophys, 2015, 71(3):1287-1291.
[8] 彭志刚, 张璐, 张旭. 裸鼠胃癌模型的相位衬度微血管成像[J]. 北京生物医学工程, 2017, 36(2):123-127.
[9] Saluja A K, Dudeja V. Relevance of animal models of pancreatic cancer and pancreatitis to human disease[J]. Gastroenterology, 2013, 144(6):1194-1198.
[10] 欧阳东, 黄鹤光. 三种鼠胰腺癌动物模型的建立[J]. 中华实验外科杂志, 2012, 29(11):2321-2322.
[11] 胡伟泽, 李志水, 冯江华, 等. 三种原位种植入胰腺癌裸鼠模型的建立及其血清代谢组学[J]. 中华肝胆外科杂志, 2016, 22(3):188-192.
[12] 何伟, 王冬青, 孙维斌, 等. 人胰腺癌细胞株PANC1裸鼠原位模型的建立及磁共振监测[J]. 中华胰腺病杂志, 2009, 9(5):328-330.
[13] Hu J, Li P, Yin X, et al. Nondestructive imaging of the internal microstructure of vessels and nerve fibers in rat spinal cord using phase-contrast synchrotronradiation microtomography[J]. J Synchrotron Radiat, 2017, 24(Pt2):482-489.
[14] 易明皓. 基于实验室光源的X射线相衬成像应用研究[D]. 广东:深圳大学, 2017.
[15] 简建波, 杨浩, 秦莉莉, 等. 肝细胞癌微观结构的X线相衬CT成像研究[J]. 中国医学影像学杂志, 2016, 24(11):869-872.
[16] Grandl S, Scherer K, Gaul A S, et al. Improved visualization of breast cancer features in multifocal carcinoma using phase-contrast and dark-field mammography:an ex vivo study[J]. Eur Radiol, 2015, 25(12):3659-3668.

同步辐射X线相衬成像对裸鼠胰腺癌的早期诊断

Early diagnosis of pancreatic cancer in a nude mice model on synchrotron radiation X-ray phase contrast imaging

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	杨敏, 燕翔, 刘锡娟, 安国, 丁慧荣, 蔡英, 葛会争. 人肺癌细胞原位移植瘤小鼠模型免疫细胞的多色流式细胞术检测方法的建立[J]. 首都医科大学学报, 2023, 44(1): 99-106.
[2]	黄琪, 周亦伦, 孙雪峰. 共享外周循环对糖尿病肾病小鼠肾损伤的影响[J]. 首都医科大学学报, 2022, 43(5): 674-679.
[3]	张慧博, 杨晓旭, 刘欣圆, 顾华, 王双坤, 杨旗. 磁共振影像对脑静脉窦血栓形成的诊断价值研究[J]. 首都医科大学学报, 2022, 43(1): 67-73.
[4]	张越, 卢洁. 颈动脉粥样硬化斑块稳定性评估正电子发射断层扫描/磁共振研究进展[J]. 首都医科大学学报, 2021, 42(1): 31-36.
[5]	张晨, 刘子歌, 宋国瑞, 李燕, 陈德胜, 程萌旗. 钛颗粒诱导骨溶解的不同动物模型形态学研究[J]. 首都医科大学学报, 2021, 42(1): 37-42.
[6]	郑爽爽, 高志远, 宋双双, 何悦明, 卢洁. BOLD MRI评价糖尿病患者尿微量白蛋白及氧合水平的研究[J]. 首都医科大学学报, 2021, 42(1): 125-130.
[7]	杨阳, 康骅, 赵菁, 凌煜玮, 赵烨, 王亚军, 戎冬冬. 术前乳腺MRI对乳腺癌保乳手术切缘评估的回顾性分析[J]. 首都医科大学学报, 2020, 41(6): 943-953.
[8]	李妍. 守护“记忆之魂”的中国痴呆临床研究引领者——贾建平教授[J]. 首都医科大学学报, 2020, 41(5): 822-827.
[9]	白志杰, 王新胜, 马洪顺, 李波. mpMRI与超声融合导航技术在前列腺靶向穿刺的临床研究[J]. 首都医科大学学报, 2020, 41(4): 631-635.
[10]	张闻平, 吴静, 任彦军, 常莹, 张雪娟, 马佳, 杜岚, 杨敬春. 直肠腔内超声与磁共振诊断直肠肿瘤的比较研究[J]. 首都医科大学学报, 2020, 41(4): 641-646.
[11]	刘鑫垚, 马超, 贾竑晓. 精神分裂症认知损害动物模型的研究现状[J]. 首都医科大学学报, 2020, 41(1): 50-54.
[12]	马登磊, 张旭, 罗艺, 黄蕊, 李雅莉, 李林, 张兰. 微管相关蛋白tau转基因细胞和动物模型的建立及tau蛋白病变表征[J]. 首都医科大学学报, 2019, 40(4): 582-587.
[13]	张媛媛, 葛洋, 安广宇. 双肾上腺素样激酶1不同剪接体通过激活JNK通路增强胰腺癌细胞的增生能力[J]. 首都医科大学学报, 2019, 40(2): 179-185.
[14]	杨硕, 曹金鑫, 王明刚, 卢静, 陈杰, 申英末, 邹振玉, 刘雨辰. 去腹壁运动神经支配建立兔腹壁薄弱模型[J]. 首都医科大学学报, 2019, 40(2): 282-285.
[15]	李敏, 蒋涛. 增强MRI对睾丸肿瘤及肿瘤样病变病理分型的诊断价值[J]. 首都医科大学学报, 2019, 40(2): 305-311.