茶叶中咖啡因和儿茶素类物质的提取及含量测定

doi:10.3969/j.issn.1006-7795.2019.05.014

首都医科大学学报 ›› 2019, Vol. 40 ›› Issue (5): 738-743.doi: 10.3969/j.issn.1006-7795.2019.05.014

茶叶中咖啡因和儿茶素类物质的提取及含量测定

邵建群, 李青原, 王玉记, 张枫

首都医科大学药学院实验教学中心, 北京 100069

收稿日期:2019-05-14 出版日期:2019-09-21 发布日期:2019-12-16
通讯作者: 张枫 E-mail:zhangf@ccmu.edu.cn
基金资助:
北京市教育委员会人才培养质量建设项目（PXM2017_014226_000073），首都医科大学2019年度教学建设性项目（119002）。

Extraction process and determination of caffeine and catechins from tea

Shao Jianqun, Li Qingyuan, Wang Yuji, Zhang Feng

Experimental Teaching Center, School of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, China

Received:2019-05-14 Online:2019-09-21 Published:2019-12-16
Supported by:
This study was supported by Beijing Municipal Education Commission for construction of talent training quality(PXM2017_014226_000073), 2019 Capital Medical University for construction of teaching (119002).

摘要/Abstract

摘要： 目的研究加热和超声法同时提取茶叶中的咖啡因（caffeine，CAF）、表没食子儿茶素没食子酸酯（epigallocatechin gallate，EGCG）、表儿茶素没食子酸酯（epicatechin gallate，ECG）、表儿茶素（epicatechin，EC）的最佳提取工艺。方法采用高效液相色谱仪为检测手段，分别研究乙醇浓度、提取时间、料液比对用加热法和超声法提取茶叶4种有效成分的影响。结果加热浸提法的工艺是乙醇体积分数为65%，料液比为1∶25，加热85℃搅拌提取30 min；超声波浸提的工艺是乙醇体积分数为65%，料液比为1∶25，室温超声提取60 min。结论两种提取工艺提取效率相当，4种有效成分在提取液中的含量从高到底的顺序为EC、EGCG、CAF、ECG；儿茶素类物质EC、EGCG、ECG在绿茶、花茶中的含量显著高于红茶和普洱茶。

关键词: 茶叶, 咖啡因, 儿茶素, 加热提取, 超声提取

Abstract: Objective Research on heating and ultrasonic method optimum extraction technology of caffeine (CAF),epigallocatechin gallate (EGCG), epicatechin gallate (ECG),epicatechin (EC) in tea. Methods The amounts of effective components from tea were determined by high performance liquid chromatography (HPLC) and the ethanol concentration, extraction time and solid-liquid ratio as the factors affecting were studied. Results Heating extraction process was ethanol concentration of 65%, solid-liquid ratio of 1:25 and extraction for 30 min at 85℃. Ultrasonic extraction process was ethanol concentration of 65%, solid-liquid ratio of 1:25 and ultrasonic for 60 min at room temperature. Conclusion The results showed that two kinds of extraction technology had the same extraction efficiency and four kinds of effective components in the extract content from the high to the end order were EC, EGCG, CAF and ECG. Catechin material in EC, EGCG and ECG in green tea and scented tea were significantly higher than that of black tea and puer tea.

Key words: tea, caffeine, catechins, heating extraction, ultrasonic extraction

中图分类号:

R284

邵建群, 李青原, 王玉记, 张枫. 茶叶中咖啡因和儿茶素类物质的提取及含量测定[J]. 首都医科大学学报, 2019, 40(5): 738-743.

Shao Jianqun, Li Qingyuan, Wang Yuji, Zhang Feng. Extraction process and determination of caffeine and catechins from tea[J]. Journal of Capital Medical University, 2019, 40(5): 738-743.

参考文献

[1] 王丽丽,陈键,宋振硕,等. 茶叶中茶多酚检测方法研究进展[J]. 茶叶科学技术,2013(4):6-12.
[2] 焦婵媛,景春晖,员小婷,等. 表儿茶素及其衍生物表儿茶素没食子酸酯通过MAPK-ERK44/42通路抑制前列腺间质细胞增殖[J].天津中医药,2017,34(3):200-203.
[3] 陈星卉.表儿茶素没食子酸酯抑菌活性的研究[J]. 黑龙江医药科学, 2017, 40(6):129-130,132.
[4] 蔡轶,魏沁,蔡跃鹏,等. 表儿茶素没食子酸酯对人鼻咽癌C666-1细胞凋亡的影响[J].华南师范大学学报:自然科学版, 2015,47(4):103-107.
[5] 雷梦侠,王丹丹,于飞,等. 表没食子儿茶素没食子酸酯相关肿瘤临床试验研究进展[J].中国肿瘤临床,2018,45(2):97-102.
[6] Makena P S, Chung K T. Effects of various plant polyphenols on bladder carcinogen benzidine-induced mutagenicity[J]. Food Chem Toxicol, 2007, 45(10):1899-1909.
[7] 黄鸿,夏晓艳,赵余庆. 茶叶中茶氨酸、咖啡因和茶碱检测方法的研究进展[J]. 沈阳药科大学学报,2018,35(8):696-706.
[8] 侯倩倩,曹雪姣,王嘉宝,等. 咖啡因预防阿尔兹海默病作用机制的研究[J]. 现代生物医学进展,2017,17(23):4452-4455.
[9] 刘洋,董文斌.咖啡因对早产儿支气管肺发育不良的预防作用[J].中国当代儿科杂志,2018,20(7):598-602.
[10] 刘寒旸,周艳,龚宇,等. 咖啡因在肥胖、糖尿病和肿瘤中的研究进展[J]. 医学综述,2016,22(5):928-932.
[11] 浦周芳,韩丽琴,曹宏梅.茶叶中茶多酚提取及含量测定的研究[J].食品研究与开发,2013,34(17):83-85.
[12] 闫洪军,郑择,李涵汶,等. 茶叶中咖啡因的提取及条件优化[J]. 安徽化工,2018,44(4):36-37.
[13] Sökmen M, Demir E, Alomar S Y. Optimization of sequential supercritical fluid extraction (SFE) of caffeine and catechins from green tea[J]. J Supercrit Fluids, 2018, 133, 171-176.
[14] 陈键,王丽丽,宋振硕.茶叶色素研究进展[J]. 茶叶科学技术,2013(4):13-16.
[15] 胡雅琼,罗理勇,曾亮.茶叶提取物对帕金森病的防治作用研究进展[J].中草药,2014,45(9):1342-1348.
[16] Mancini E, Beglinger C, Drewe J, et al. Green tea effects on cognition, mood and human brain function:a systematic review[J]. Phytomedicine, 2017, 34:26-37.
[17] 申雯,黄建安,李勤,等. 茶叶主要活性成分的保健功能与作用机制研究进展[J].茶叶通讯,2016.43(1):8-13.
[18] 高飞虎,李雪,张雪梅,等.茶叶的主要功效及在食品中的应用研究进展[J].南方农业,2018,12(28):38-40.

茶叶中咖啡因和儿茶素类物质的提取及含量测定

Extraction process and determination of caffeine and catechins from tea

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics

本文评价

[1]	扈金萍;闫淑莲;唐静成;张锦楠. Cocktail探针药物评价姜黄对肝细胞色素P₄₅₀酶的影响[J]. 首都医科大学学报, 2004, 25(4): 427-430.
[2]	崔秀玉;吕国蔚. 麻醉与兴奋小鼠急性重复缺氧耐受性的变化[J]. 首都医科大学学报, 1994, 15(1): 1-4.