关键词: sRAGE, 心肌缺血/再灌注损伤, 蛋白质组学, 蛋白变化, 功能

Abstract: Objective  To investigate the effects of soluble receptor for advanced glycation end products (sRAGE) on the changes and functions of myocardial tissue proteins in mice during myocardial ischemia/reperfusion(I/R). Methods  Establish the myocardial I/R model using cardiac-specific sRAGE transgenic mice, and apply proteomic methods to detect the types and levels of protein expression in myocardial tissue. Results  Compared with the I/R+sRAGE KI^fl/fl group, the I/R+sRAGE CKI group had 59 upregulated proteins and 42 downregulated proteins in myocardial tissue. The volcano plot showed that the significantly upregulated proteins were lghg1, lgh2b, Mcm7, and Nifk, and the significantly downregulated proteins were Abca7, Colla2, Ablim1, Crebrf, and Kcp, respectively. The subcellular localization results showed that the proteins with significant changes were mainly distributed in the nucleus, cytoplasm, extracellular space, plasma membrane, endoplasmic reticulum, cytoskeleton, and others. The results of functional enrichment showed that the significantly changed proteins were mainly involved in regulating signal transduction, cell motility, metabolism, infectious diseases, tumors and the immune system. Conclusions  RAGE can inhibit myocardial I/R injury by increasing or decreasing target proteins involved in regulating intracellular and extracellular signal transduction processes following myocardial I/R.

Key words: sRAGE, myocardial ischemia-reperfusion injury, proteomics, protein change, function