GDE1参与2型慢性鼻窦炎伴鼻息肉脂代谢通路激活的研究

doi:10.3969/j.issn.1006-7795.2026.04.013

首都医科大学学报 ›› 2026, Vol. 47 ›› Issue (4): 722-729.doi: 10.3969/j.issn.1006-7795.2026.04.013

GDE1参与2型慢性鼻窦炎伴鼻息肉脂代谢通路激活的研究

王麒淇^1,2,郭雨石^1,2,李艳^1,2*

1.首都医科大学附属北京同仁医院耳鼻咽喉头颈外科，北京 100730；2.北京市耳鼻咽喉科研究所，过敏性疾病北京实验室（北京市教育委员会），慢性鼻病新药及诊断技术研发北京市重点实验室，北京 100005

收稿日期:2026-04-07 修回日期:2026-05-28 出版日期:2026-08-21 发布日期:2026-07-26
通讯作者: 李艳 E-mail:liyanok@126.com
基金资助:
首都卫生发展科研专项-青年优才项目（2024-4-1092）,国家自然科学基金项目（82201253）,北京市卫生健康委员会改革与发展专项，北京市卫生健康委员会耳鼻咽喉科慢病创新机制研究项目。

GDE1 contributes to lipid metabolic pathway dysregulation in type 2 chronic rhinosinusitis with nasal polyps

Wang Qiqi^1,2, Guo Yushi^1,2, Li Yan^1,2*

1.Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China；2.Beijing Laboratory of Allergic Diseases, Beijing Municipal Education Commission and Beijing Key Laboratory of New Medicine and Diagnostic Technology Research for Nasal Disease, Beijing Institute of Otolaryngology, Beijing 100005, China

Received:2026-04-07 Revised:2026-05-28 Online:2026-08-21 Published:2026-07-26
Supported by:
This study was supported by Capital‘s Funds for Health Improvement and Research (2024-4-1092), National Natural Science Foundation of China (82201253), Special Project for Reform and Development of Beijing Municipal Health Commission, Research Project on Innovative Mechanisms of Chronic Otorhinolaryngological Diseases of Beijing Municipal Health Commission.

摘要/Abstract

摘要： 目的揭示慢性鼻窦炎伴鼻息肉（chronic rhinosinusitis with nasal polyps，CRSwNP）的免疫细胞浸润特征和脂代谢激活情况。方法从基因表达综合（Gene Expression Omnibus，GEO）数据库中选取8个包含正常鼻黏膜与CRSwNP组织测序结果的数据集。去除批次效应后，应用加权基因共表达网络分析（Weighted Gene Co-expression Network Analysis，WGCNA）识别与CRSwNP相关的关键模块，并分析CRSwNP组织与对照组的差异基因，对关键模块内的差异基因进行功能富集分析。通过xCell免疫浸润分析软件分析CRSwNP组织免疫细胞浸润特征；重点探究2型炎症不同浸润程度对CRSwNP代谢通路激活的影响，筛选关键基因后通过实时荧光聚合酶链式反应（real-time fluorescence polymerase chain reaction，RT-PCR）验证其表达差异。结果与对照组相比，CRSwNP组织中的午夜蓝模块差异基因主要参与代谢过程调控，其中免疫浸润分析表明，2型辅助性T（T helper 2，Th2）细胞在CRSwNP组织中浸润更显著，且高2型炎症浸润的CRSwNP组织中脂代谢通路显著富集，对关键基因进行RT-PCR验证，发现甘油磷酸二酯磷酸二酯酶1（glycerophosphodiester phosphodiesterase 1，GDE1)在2型CRSwNP组织中表达水平上调。结论 GDE1可能通过调控2型CRSwNP的脂质代谢通路激活水平影响疾病进展。

关键词: 慢性鼻窦炎, 免疫细胞浸润, 生物信息学, 2型炎症反应, 脂代谢, 甘油磷酸二酯磷酸二酯酶1

Abstract: Objective To elucidate the immune cell infiltration characteristics and lipid metabolic activation in chronic rhinosinusitis with nasal polyps (CRSwNP). Methods Eight datasets from the Gene Expression Omnibus（GEO） database containing bulk-RNA sequencing results of normal nasal mucosa and CRSwNP tissues were selected. After batch effect correction, Weighted Gene Co-expression Network Analysis (WGCNA) was performed to identify key modules associated with CRSwNP. Additionally, differentially expressed genes (DEGs) between CRSwNP tissues and the control group were analyzed, followed by functional enrichment analysis of the DEGs within the key modules. XCell analysis was used to characterize immune cell infiltration in CRSwNP tissues. The impact of varying Th2 cell infiltration levels on activation of lipid metabolic pathway in CRSwNP was investigated, and key genes were screened for validation using real-time fluorescence polymerase chain reaction (RT-PCR). Results Compared to the controls, DEGs in the midnight blue module of CRSwNP tissues were primarily involved in the regulation of metabolic processes. Immune infiltration analysis revealed more prominent Th2 cell infiltration in CRSwNP tissues, and lipid metabolic pathways were significantly enriched in high-type 2 CRSwNP tissues. The expression of the key gene GDE1 was upregulated in type 2 CRSwNP tissues as measured by RT-PCR.Conclusion GDE1 may influence disease progression by modulating the activation level of lipid metabolic pathways in type 2 CRSwNP.

Key words: chronic rhinosinusitis, immune cell infiltration, bioinformatics, type 2 inflammatory response, lipid metabolism, glycerophosphodiester phosphodiesterase 1

中图分类号:

R765.4+1

王麒淇, 郭雨石, 李艳. GDE1参与2型慢性鼻窦炎伴鼻息肉脂代谢通路激活的研究[J]. 首都医科大学学报, 2026, 47(4): 722-729.

Wang Qiqi, Guo Yushi, Li Yan. GDE1 contributes to lipid metabolic pathway dysregulation in type 2 chronic rhinosinusitis with nasal polyps[J]. Journal of Capital Medical University, 2026, 47(4): 722-729.

参考文献

［1］中华耳鼻咽喉头颈外科杂志编辑委员会鼻科组, 中华医学会耳鼻咽喉头颈外科学分会鼻科学组. 慢性鼻窦炎诊断和治疗指南(2024)［J］. 中华耳鼻咽喉头颈外科杂志, 2025, 60(3): 221－249.
［2］Wang M, Li Y, Li J Y, et al. New insights into the endotypes of chronic rhinosinusitis in the biologic era［J］. J Allergy Clin Immunol, 2025, 156(1): 51－60.
［3］Park J, Jang J Y, Kim J H, et al. SLC27A2 marks lipid peroxidation in nasal epithelial cells driven by type 2 inflammation in chronic rhinosinusitis with nasal polyps［J］. Exp Mol Med, 2025, 57(4): 856－871.
［4］Li Y, Liang S, Wang H Q, et al. Type 2 inflammation-biased arachidonic acid metabolite regulates mucosal remodeling of chronic rhinosinusitis with nasal polyps［J］. Allergy, 2026, 81(5): 1680－1696.
［5］中华耳鼻咽喉头颈外科杂志编辑委员会鼻科组, 中华医学会耳鼻咽喉头颈外科学分会鼻科学组. 中国慢性鼻窦炎诊断和治疗指南(2018)［J］. 中华耳鼻咽喉头颈外科杂志, 2019, 54(2): 81－100.
［6］蓝凤, 赵丽敏, 杨萌哲, 等. 2型和非2型慢性鼻窦炎不伴鼻息肉的临床特征及组织重塑分析［J］. 中国耳鼻咽喉头颈外科, 2022, 29(5): 304－308.
［7］蓝凤, 王麒淇, 张罗. 慢性鼻窦炎伴鼻息肉鼻黏膜上皮细胞基因转录组分析［J］. 中华耳鼻咽喉头颈外科杂志, 2021, 56(10): 1066－1072.
［8］史剑波, 程雷, 李华斌, 等. 2型慢性鼻窦炎临床诊疗意见书［J］. 中华耳鼻咽喉头颈外科杂志, 2024, 59(10): 990－1001.
［9］Lan F, Li J Z, Miao W X, et al. GZMK-expressing CD8⁺ T cells promote recurrent airway inflammatory diseases［J］. Nature, 2025, 638(8050): 490－498.
［10］Guo C L, Wang C S, Wang Z C, et al. Granzyme K⁺CD8⁺ T cells interact with fibroblasts to promote neutrophilic inflammation in nasal polyps［J］. Nat Commun, 2024, 15(1): 10413.
［11］Chapurin N, Soler Z M, Khan S, et al. Type 2 biomarkers in olfactory cleft mucus correlate with SNOT-22 in chronic rhinosinusitis Independent of nasal polyp status［J］. Int Forum Allergy Rhinol, 2025, 15(4): 411－419.
［12］Schirinzi T, Lattanzi R, Maftei D, et al. Substance P and prokineticin-2 are overexpressed in olfactory neurons and play differential roles in persons with persistent post-COVID-19 olfactory dysfunction［J］. Brain Behav Immun, 2023, 108: 302－308.
［13］Perner C, Flayer C H, Zhu X P, et al. Substance P release by sensory neurons triggers dendritic cell migration and initiates the type-2 immune response to allergens［J］. Immunity, 2020, 53(5): 1063－1077.e7.
［14］Maghni K, Taha R, Afif W, et al. Dichotomy between neurokinin receptor actions in modulating allergic airway responses in an animal model of helper T cell type 2 cytokine-associated inflammation［J］. Am J Respir Crit Care Med, 2000, 162(3): 1068－1074.
［15］Nagasaki T, Schuyler A J, Zhao J M, et al. 15LO1 dictates glutathione redox changes in asthmatic airway epithelium to worsen type 2 inflammation［J］. J Clin Invest, 2022, 132(1): e151685.
［16］Wang W Q, Xu Y, Wang L, et al. Single-cell profiling identifies mechanisms of inflammatory heterogeneity in chronic rhinosinusitis［J］. Nat Immunol, 2022, 23(10): 1484－1494.［17］Zheng B, Berrie C P, Corda D, et al. GDE1/MIR16 is a glycerophosphoinositol phosphodiesterase regulated by stimulation of G protein-coupled receptors［J］. Proc Natl Acad Sci U S A, 2003, 100(4): 1745－1750.
［18］Lee E J, Hwang H J, Jung C M, et al. The relationship between chronic rhinosinusitis and metabolic syndrome［J］. Am J Rhinol Allergy, 2017, 31(4): 222－227.
［19］Yagyu H, Kiuchi M, Sasaki A, et al. Lipolysis-microlipophagy cascade regulated by adipose triglyceride lipase drives pathogenic adaptive type 2 immunity［J］. Sci Immunol, 2025, 10(112): eadp0849.
［20］Karagiannis F, Masouleh S K, Wunderling K, et al. Lipid-droplet formation drives pathogenic group 2 innate lymphoid cells in airway inflammation［J］. Immunity, 2020, 52(4): 620－634.e6.
［21］Chen S Y, Aisyah R, Honda A, et al. Overexpression of GDE1 decreases glycerophosphoinositol in intestinal epithelial cells in vivo［J］. Biosci Biotechnol Biochem, 2025, 89(9): 1312－1319.
［22］Simon G M, Cravatt B F. Anandamide biosynthesis catalyzed by the phosphodiesterase GDE1 and detection of glycerophospho-N-acyl ethanolamine precursors in mouse brain［J］. J Biol Chem, 2008, 283(14): 9341－9349.

[1]	赵越, 阮祥燕, 王月姣, 谢丹, 金婧, 田玄玄, 张煦, 张娥, 朱建国, 张政. 多囊卵巢综合征患者关键调控分子表达与代谢紊乱的相关性研究[J]. 首都医科大学学报, 2026, 47(4): 643-649.
[2]	李妍秋, 阮祥燕, 牧青, 蒋玲玲, 张明珍, Alfred O. Mueck. 屈螺酮炔雌醇片和地屈孕酮治疗对超重多囊卵巢综合征患者体成分与脂代谢的影响[J]. 首都医科大学学报, 2026, 47(4): 665-672.
[3]	杨婧兴, 赵妍, 陈陆泉, 蔡超, 王向东. 基于网络药理学探究黄芩治疗慢性鼻窦炎的潜在作用机制[J]. 首都医科大学学报, 2024, 45(2): 252-261.
[4]	王泽铖, 阮祥燕, 李婧, 蒋玲玲, Alfred O.Mueck. 医源性早发性卵巢功能不全对脂代谢的影响[J]. 首都医科大学学报, 2023, 44(4): 535-539.

GDE1参与2型慢性鼻窦炎伴鼻息肉脂代谢通路激活的研究

GDE1 contributes to lipid metabolic pathway dysregulation in type 2 chronic rhinosinusitis with nasal polyps

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 4

编辑推荐

Metrics

本文评价