鼠胚心肌细胞体外培养模型的建立

首都医科大学学报 ›› 2006, Vol. 27 ›› Issue (5): 604-606.

鼠胚心肌细胞体外培养模型的建立

高丽芳¹, 李勇², 陈祥贵²

1. 首都医科大学食品药品安全评价中心;2. 北京大学公共卫生学院营养与食品卫生学系

收稿日期:2005-11-23 修回日期:1900-01-01 出版日期:2006-10-24 发布日期:2006-10-24

The Model of Cultured Mice Embryonic Cardiomyocytes in Vitro

Gao Lifang¹, Li Yong², Chen Xianggui²

1. Center for Food and Drug Safety Assessment, Capital Medical University;2. Department ofNutrition and Food Hygiene, School of Public Health, Peking University

Received:2005-11-23 Revised:1900-01-01 Online:2006-10-24 Published:2006-10-24

摘要/Abstract

摘要： 目的建立经济适用的小鼠胚胎心肌细胞的分离、培养和鉴定方法.方法取不同胚龄小鼠心脏,采用不同方法进行消化、接种、体外增生、鉴定.结果用0.125%胰蛋白酶和0.02%EDTA混合消化液,短时间、分步消化孕12 d鼠胚的心脏.将获得的细胞悬液接种于胎牛血清包被的培养皿中,采用pH值为7.2～7.4的HAM'S F12加20%胎牛血清培养液进行培养,所获得的细胞经PAS反应及免疫组化鉴定显示约80%的细胞为心肌细胞. 结论建立的鼠胚心肌细胞培养模型可进行胚胎心脏发育毒性的体外研究.

关键词: 心肌细胞, 体外原代培养

Abstract: Objective To establish the model of primary cultured mice embryonic cardiomyocytes,which is adapted to isolation,culture and identification. Methods To isolate hearts from different day gestational age mice,then digested,seeded,proliferation in vitro and identified in different ways. Results Repeat digestion with 0.125% trypsin and 0.02% EDTA,then in the form of cell suspension seeded in tissue culture plate precoated with fetal bovine serum,and added with Ham F12 containing 20% FBS(pH 7.2⁷.4),and finally identified cells with PAS staining and immunocytochemical methods,which proved the rate between cardiomyocytes and cultured cells was about 80%.Conclusion The model of cultured mice embryonic cardiomyocytes has highly effective.

Key words: myocardial cells, primary cultured

中图分类号:

R329.2

高丽芳;李勇;陈祥贵. 鼠胚心肌细胞体外培养模型的建立[J]. 首都医科大学学报, 2006, 27(5): 604-606.

Gao Lifang;Li Yong;Chen Xianggui. The Model of Cultured Mice Embryonic Cardiomyocytes in Vitro[J]. Journal of Capital Medical University, 2006, 27(5): 604-606.

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