脊髓损伤致自发痛动物模型的建立

首都医科大学学报 ›› 2005, Vol. 26 ›› Issue (6): 670-672.

脊髓损伤致自发痛动物模型的建立

戴红¹, 胥少汀², 肖忠新¹, 张洪³, 谢作磊⁴, 张立军³, 陈迎朝², 张立仁², 王元身⁵, 戴珂⁶

1. 首都医科大学公共卫生与家庭医学学院康复医学教研室;2. 北京军区总医院骨科;3. 唐山市截瘫疗养院;4. 北京协和医科大学基础医学研究所生理室;5. 首都医科大学神经科学研究所;6. 北京神经外科研究所神经病理室

收稿日期:2005-11-08 修回日期:1900-01-01 出版日期:2005-12-24 发布日期:2005-12-24

An Animal Model of Spinal Cord Injury-induced Spontaneous Pain

Dai Hong¹, Xu Shaoting², Xiao Zhongxin¹, Zhang Hong³, Xie Zuolei⁴, Zhang Lijun³, Chen Yingchao², Zhang Liren², Wang Yuanshen⁵, Dai Ke⁶

1. Department of Rehabilitation Medicine, School of Public Health and Family Medicine, Capital University of Medical(Sciences);2. Department of Orthopedics, Beijing Military General Hospital;3. Paraplegics Sanatorium of Tang Shan;4. (Department) of Physiology, Institute of Basic Medical Sciences, Peking Union Medical College;5. Beijing Institute of Neuroscience, Capital University of Medical Sciences;6. Department of neuropathology, Beijing Neurosurgical Institute

Received:2005-11-08 Revised:1900-01-01 Online:2005-12-24 Published:2005-12-24

摘要/Abstract

摘要： 目的建立脊髓损伤所致自发痛动物模型。方法健康成年Wistar大鼠30只分为2组,用WADE法复制脊髓L₂节段3Ncm(G1组)和2Ncm(G2组)损伤,分别在损伤前、后15min在L₁节段记录体感诱发电位,分别在损伤前、后4、8、12、16、24h记录大鼠躯干部皮肤对轻压、轻触觉刺激反应和前足对弗莱毛轻压的抬足率等痛阈变化,并每日观察大鼠自发痛行为学的变化,持续10个月。结果 2组动物均出现痛超敏和明显的自发痛现象,其中G1组的严重自发痛(自噬)的发生率为50%,接近人类脊髓损伤(spinalcordinjury,SCI)所致的中枢性疼痛(centralpain,CP)中自发痛的比例。结论用WADE法复制大鼠3NcmSCI自发痛的模型具有良好的可重复性,其自发痛症状与人类SCI的CP接近,为研究自发痛发生发展机制提供了实验条件。

关键词: 脊髓损伤, 自发痛, 动物模型, WADE法

Abstract: Objective To establish a practical and reliable animal model of spontaneous pain after spinal injury.Methods 30 female Wistar rats were divided into 2 groups: G1 and G2.The injury was induced in L₂ segment of spinal cord using₃ Ncm (G1) and 2 Ncm (G2) by WADEmethod, respectively.SEP (somatosensory evoked potential) was recorded in L₁ before injury induction and 15 min after injury.The pain threshold of rats in trunk to mechanical gently pressure, gently tactile and rate of forepaws'withdrawing was assessed using von Frey hair just before and after 4 h, 8 h, 12 h, 16 h, 24 h injury everyday for about 10 months.Behavior activities of spontaneous pain were also determined everyday.Results Two groups exhibited typical allodynia phenomena.The pain threshold before and after injury has decreased significantly (P<0.05～0.01) .The obvious spontaneous pain could be observed.The proportion of serious spontaneous pain (autotomy) in G1 group is 50%, which was near to that of the clinical observations.Conclusion SCIof 3 Ncm in rat model by WADEmethod has an excellent repeatability. (It's) spontaneous pain symptom is near to that of CPin SCIpatients.The animal model will be useful for SCIpain studies.

Key words: spinal cord injury, spontaneous pain, animal model, WADE method

中图分类号:

R332

戴红;胥少汀;肖忠新;张洪;谢作磊;张立军;陈迎朝;张立仁;王元身;戴珂. 脊髓损伤致自发痛动物模型的建立[J]. 首都医科大学学报, 2005, 26(6): 670-672.

Dai Hong;Xu Shaoting;Xiao Zhongxin;Zhang Hong;Xie Zuolei;Zhang Lijun;Chen Yingchao;Zhang Liren;Wang Yuanshen;Dai Ke. An Animal Model of Spinal Cord Injury-induced Spontaneous Pain[J]. Journal of Capital Medical University, 2005, 26(6): 670-672.

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