关键词: 钙调蛋白, 拮抗剂, Tenon囊, 成纤维细胞

Abstract: Objective To evaluate the effects of calmodulin antagonist W-7 on the proliferation of in vitro cultured human Tenon's capsule fibroblast cells for finding new antiproliferative drugs applied for the glaucoma filtration surgery. Methods Human Tenon's capsule fibroblast cells were cultured in DMEM medium containing 10% fetal bovine serum at 37 ℃,5% CO₂. For growth inhibition assays, cells lie in logarithm growth period were seeded into 96 well plate, and treated with W-7 and MMC, the inhibitory effect was determined by MTT(tetrazolium bromide) method. The mitochondrial metabolic ability of the drugs treated cells was also examined by MTT assays. Results W-7 caused significant inhibition effect of fibroblast proliferation in dose and time dependent manner, which were perhaps the causes of cell apoptosis in human Tenon's capsule fibroblast cells. The MTT assay showed that W-7 treated cells had mitochondrial metabolic ability while MMC treated cells lost this ability. While the cell membrane integrity of W-7 treated cells was superior to MMC treated cells, and it may cause slight inflammatory reaction. Conclusion There is a good prospect of W-7 for clinical application.

Key words: CaM, antagonist, Tenon's capsule, fibroblasts