黄芪碱化合物HDTIC-1和2对衰老相关基因p16和p21表达的影响

首都医科大学学报 ›› 2007, Vol. 28 ›› Issue (2): 145-149.

黄芪碱化合物HDTIC-1和2对衰老相关基因p16和p21表达的影响

王培昌¹, 张建², 张宗玉³, 童坦君³

1. 首都医科大学宣武医院检验科;2. 首都医科大学宣武医院胸心血管外科;3. 北京大学衰老研究中心

收稿日期:2007-01-18 修回日期:1900-01-01 出版日期:2007-04-24 发布日期:2007-04-24

The Effects of HDTIC Compounes Isolated from Astragali Radix on the Expression of p16 and p21

Wang Peichang¹, Zhang Jian², Zhang Zongyu³, Tong Tanjun³

1. Department of Clinical Laboratory, Xuanwu Hospital, Capital Medical University;2. Department of Thoracic and CardiovascularSurgery, Xuanwu Hospital, Capital Medical University;3. Research Center of Aging, Health and Science Center of Peking University

Received:2007-01-18 Revised:1900-01-01 Online:2007-04-24 Published:2007-04-24

摘要/Abstract

摘要：

目的观察黄芪碱化合物HDTIC-1和2对人胚肺二倍体成纤维细胞(2BS)p16、p21表达的影响,以探讨其延缓复制性衰老的分子机制。方法用RT-PCR、Western blot法分析HDTIC化合物培养的2BS细胞及对照细胞p16、p21表达水平。结果 1)HDTIC培养的2BS细胞在45代龄未见p16表达,在56代龄p16虽在mRNA水平有弱表达,但在蛋白质水平未见表达,而老龄对照细胞p16表达显著;2)HDTIC培养的2BS细胞在中年代龄p21有弱表达,在老龄细胞p21表达明显,HDTIC培养的2BS细胞与对照细胞p21表达水平差异无统计学意义。结论 HDTIC化合物对2BS细胞p21表达无显著影响,但显著抑制p16的基因表达,这可能是HDTIC化合物延缓2BS细胞复制性衰老的主要分子机制之一。

关键词: 黄芪, 二倍体成纤维细胞, 基因表达

Abstract:

Objective To investigate the effects of HDTIC-1 and HDTIC-2 isolated from Astragali Radix on the expression of p16 and p21 of human fetal lung diploid fibroblasts(2BS) so as to explore the mechanism of HDTIC delaying replicative senescence. Methods To analyse the expression levels of p16 and p21 of 2BS cells cultured with DMEM containing HDTIC by RT-PCR and western blot. Results 1)There was no expression of p16 in PD 45 2BS cells incubated with HDTIC.In PD 56 2BS cells cultured with HDTIC, there was a weak mRNA expression of p16, whereas, the protein expression of p16 was not observed.However,an obvious expression of p16 was seen in the control senescent cells. 2)For the cells cultured with HDTIC,there was a weak expression of p21 in the middle age and a strong expression of p21 in the senescent cells.Additionally,no difference of p21 level between the HDTIC-cultured cells and the control cells was seen. Conclusion There was no effect of HDTIC compounds on the expression of p21.But p16 expression of 2BS cells was strongly inhibited by HDTIC,which may contribute to their delaying replicative senescence.

Key words: astragali radix, diploid fibroblast, gene expression

中图分类号:

R962
Q756

王培昌;张建;张宗玉;童坦君. 黄芪碱化合物HDTIC-1和2对衰老相关基因p16和p21表达的影响[J]. 首都医科大学学报, 2007, 28(2): 145-149.

Wang Peichang;Zhang Jian;Zhang Zongyu;Tong Tanjun. The Effects of HDTIC Compounes Isolated from Astragali Radix on the Expression of p16 and p21[J]. Journal of Capital Medical University, 2007, 28(2): 145-149.

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