关键词: 重组人生长激素, 肿瘤坏死因子-&alpha, BALB/C小鼠, 急性肝坏死

Abstract:

Objective To investigate the histologic effect of recombinant human growth hormone (rhGH) on acute hepatic necrosis in mouse model which induced by tumor necrotic factor-α (TNF-α)+D-galactose(D-Gal). Methods Mouse model with acute hepatic necrosis was created. Fifty male BALB/C mice were randomly divided into 5 equal groups, which were blank-control group(A), model-control group(B) and experimental groups(C, Dand E), respectively. D-Gal+TNF-α were intraperitoneally administered to every mouse in group B. In experimental groups, D-Gal+TNF-α in the same dosage as that in group Band varied dosage of rhGH (dosage: C<D<E) were intraperitoneally given to every mouse. Every one in group Awere given the same volume of normal saline. Once any one of all mice was found on the brink of death, all mice was killed and a piece of liver tissue of every mouse was taken for light microscopy. At the same time, the pieces of liver tissues from the mice (one selected randomly from every group) were taken for electron microscope observation. Blood samples of every mouse in group Aand Bwere taken for ALTand ASTserum assessment just before the mice's being killed. Results There was significant difference between the values of serum ALTassessment in group Aand those in group B(P= 0.000), and so did the values of serum AST assessment(P=0.000) between in group Aand group B. The hepatic necrosis area percentage in group Bwere significantly different from that in group E(P=0.005), while there was no significant difference between those in group Band in group C(P= 0.303), and so did that between those in group Band in group D(P=0.020). Conclusion Recombinant human growth hormone (rhGH) can alleviate acute hepatic necrosis in mouse model induced by TNF-α+D-Gal, and its protective effect is relevant to the dosage administered.

Key words: recombinant human growth hormone, tumor necrosis factor-&alpha, BALB/C mouse, acute hepatic necrosis