首都医科大学学报 ›› 1988, Vol. 9 ›› Issue (3): 170-173.

• 论著 • 上一篇    下一篇

人体与猪体内囊尾蚴蛋白质和同工酶的初步研究

姜洪杰1, 孙铁1, 杨连雪1, 卞英华1, 谢淑萍2, 孙莉2   

  1. 1. 首都医学院寄生虫学教研室;2. 首都医学院宣武医院神经内科
  • 收稿日期:1998-03-01 修回日期:1900-01-01 出版日期:1988-07-15 发布日期:1988-07-15

Preliminary Studies on the Proteins and the Isoenzymes of the Human and Hog Cysticerci

Jiang Hongjie1, Xie Shuping1   

  1. Department of Parasitology, Capital Institute of Mediecine Department of Neurology, Beijing Xuanwu Hospital
  • Received:1998-03-01 Revised:1900-01-01 Online:1988-07-15 Published:1988-07-15

摘要: 报道人体与猪体内囊尾蚴的蛋白质组分及酯酶同工酶(EST)、乳酸脱氢酶同工酶(LDH)的带谱。人和猪脑囊尾蚴液的蛋白质PAGE区带各见10和9条,猪囊尾蚴仅有1条主带,而入囊尾蚴具有2条主带;头节囊壁蛋白质PAGE区带各见18和16条,二者带谱基本相似。肌肉皮下囊尾蚴囊液蛋白质区带各有12条,猪囊尾蚴有4条带,人囊尾蚴仅有2条主带,且迁移率也有明显差别。人体和猪体内囊尾蚴囊液的LDH酶带各有4条,其迁移率略有差异;EST各见1条酶带,酶谱基本一致。肌肉皮下囊尾蚴头节囊壁LDH各见2条,EST则见3条,其迁移率也有不同。同时将两种同工酶的活性作了比较。

关键词: 猪囊尾蚴, 蛋白质区带, 酯酶, 乳酸脱氢酶

Abstract: This paper reports the proteins and isoenzyme paterns of esterase (EST) and lactate dehdrogenase (LDH) of the human and hog cerbral cysticerci. The cystic fluid of the human and hog cysticerci gave 10 and 9 bands by PAGE respectively, and the main bands in the human were 2 and in the hog only 1. The scolex-cyst Wall of two species of hosts displaied 18 and 16 bands with similar patterns by PAGE. 12 bands appeared in the cystic fluid of muscle of the hog, but only 2 appeared in that of the human having migratory rates distinctively differences with that of the former. The cystic fluid from two species of hosts gave 4 LDH bands with slightly differences of migration and 1 EST band with identical patterns. The scolex-cyst wall of two species of hosts displaied 2 LDH bands and 3 EST bands with different rates of migration. We compared the activities of LDH and EST at the same time.

Key words: cysticerci, band of proteins, esterase, lactaxe dehydrogenase