A型肉毒毒素对脊髓α运动神经元活性的调节作用

doi:10.3969/j.issn.1006-7795.2013.02.024

首都医科大学学报 ›› 2013, Vol. 34 ›› Issue (2): 292-297.doi: 10.3969/j.issn.1006-7795.2013.02.024

A型肉毒毒素对脊髓α运动神经元活性的调节作用

高宝勤¹, 田朝霞¹, 梁云¹, 张绍东², 俞雅珍¹, 王雅洁¹, 邓亚仙¹, 田亚萍¹, 杨伟力¹, 王桂芬¹

1. 首都医科大学附属北京天坛医院儿科, 北京 100050;
2. 北京市神经外科研究所, 北京 100050

收稿日期:2012-05-07 出版日期:2013-04-21 发布日期:2013-04-17
通讯作者: 田朝霞 E-mail:tian_19732000@sina.com

Activity of α motor neuron of spinal cord modulated by botulinum toxin A

GAO Baoqin¹, TIAN Zhaoxia¹, LIANG Yun¹, ZHANG Shaodong², YU Yazhen¹, WANG Yajie¹, DENG Yaxian¹, TIAN Yaping¹, YANG Weili¹, WANG Guifen¹

1. Department of Pediatric, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China;
2. Beijing Neurosurgical Institute, Beijing 100050, China

Received:2012-05-07 Online:2013-04-21 Published:2013-04-17

摘要/Abstract

摘要：

目的探索大鼠背根神经节A型肉毒毒素注射对脊髓前角α运动神经元活性及相关肌肉运动功能的影响作用。方法40只雄性Wistar大鼠,采用数字表法随机分为4组(每组10只):A组、B组、C组、D组。于实验前1天对各组大鼠进行H反射检测及运动功能评价,后以10%水合氯醛0.3 mL/kg腹腔注射进行麻醉,无菌条件下行左侧L₄、L₅、L₆限制性椎板部分切除后暴露背根神经节。A组、B组分别以A型肉毒毒素背根神经节注射,C组注射等容积(6 μL)0.9%氯化钠注射液,D组不干预为正常对照组。各组分别于注射后1,3,7,14 d进行H反射及运动功能评定。结果 (1)H反射测定:A型肉毒毒素注射后各观察时间点,A组大鼠左后肢H反射潜伏期、波幅较注射前均明显延长、降低(P<0.05,P<0.01);B、 C、D 3组大鼠注射后各观察时间点与注射前比较H反射潜伏期及波幅差异均无统计学意义。A组大鼠A型肉毒毒素注射后各观察时间点H反射潜伏期及波幅较B、C、D组的相应观察时间点明显延长(P<0.05)和降低(P<0.01);A型肉毒毒素注射后各观察时间点B组与C组,B组与D组,C组与D组组间两两比较,H反射潜伏期及波幅差异无统计学意义(P>0.05)。(2)改良Rivlin斜板实验:A组大鼠,A型肉毒毒素注射后各观察时间点,斜板度数均较注射前为低(P<0.05);A型肉毒毒素注射后第7天,第14天,A组斜板度数显著低于B、C、D组(P<0.05,P<0.01,P<0.01);注射后不同时间点B组与C组,B组与D组,C组与D组斜板度数组间两两比较差异无统计学意义(P>0.05)。结论背根神经节A型肉毒毒素注射具有抑制脊髓反射环路,降低脊髓前角α运动神经元兴奋性的作用。

关键词: A型肉毒毒素, 背根神经节, α运动神经元

Abstract:

Objective To study the modulating effect of botulinum toxin A(BTX-A) on the α motor neurons in spinal cord anterior horn. Methods Forty Wistar rats were randomly divided into four groups: group A, group B, group C, and group D. All rats in each group were anesthetized with 10% chloraldurat (0.3 mL/kg). Dorsal root ganglion(DRG) of L₄, L₅, L₆ on the left were exposed following partial laminectomy under the condition of sterility. In Group A and group B, 7 U/kg, 4 U/kg of BTX-A was injected into DRG respectively. In Group C, DRG was injected with the same volume of physiological saline. Group D is the control group. Spinal reflex function and neuroethology were evaluated before and 1, 3, 7, 14 days after BTX-A administration. Results H reflex latency and amplitude of the left hind limb of the rats were longer(P<0.05) and lower(P<0.01) than that of before BTX-A injection in group A. There was also no significant difference of H reflex latency and amplitude in goup B, C, and D when compared before and after BTX-A injection. H reflex latency and amplitude at different time points after BTX-A injection in group A were longer and lower than those of group B, C and D(P<0.05, P<0.01) on 7 days and 14 days after BTX-A injection. There was no significant difference of H reflex latency and amplitude at different time points after BTX-A injection between goup B and C, goup B and D, group C and D(P>0.05). The degree of modified Rivlin loxotic plate test at different time points is lower when compared after and before BTX-A injection in group A(P<0.05). The degree of modified Rivlin loxotic plate test in group A is lower than that of group B, C and D at different time points after BTX-A injection(P<0.05). There was no significant difference of modified Rivlin loxotic plate test degree between group B and C, group B and D,or group C and D(P>0.05) at different time points after BTX-A injection. Conclusion DRG injection of botulinum toxin type A may fundamentally block circuit of spinal and lower excitability of the α motor neurons in spinal cord anterior horn.

Key words: botulinum toxin type A, dorsal root ganglion, &alpha, motor neurons

中图分类号:

R651.3

高宝勤, 田朝霞, 梁云, 张绍东, 俞雅珍, 王雅洁, 邓亚仙, 田亚萍, 杨伟力, 王桂芬. A型肉毒毒素对脊髓α运动神经元活性的调节作用[J]. 首都医科大学学报, 2013, 34(2): 292-297.

GAO Baoqin, TIAN Zhaoxia, LIANG Yun, ZHANG Shaodong, YU Yazhen, WANG Yajie, DENG Yaxian, TIAN Yaping, YANG Weili, WANG Guifen. Activity of α motor neuron of spinal cord modulated by botulinum toxin A[J]. Journal of Capital Medical University, 2013, 34(2): 292-297.

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A型肉毒毒素对脊髓α运动神经元活性的调节作用

Activity of α motor neuron of spinal cord modulated by botulinum toxin A

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