标本保存方法和时间对血清总二氧化碳结果的影响

doi:10.3969/j.issn.1006-7795.2014.04.011

首都医科大学学报 ›› 2014, Vol. 35 ›› Issue (4): 438-442.doi: 10.3969/j.issn.1006-7795.2014.04.011

标本保存方法和时间对血清总二氧化碳结果的影响

杜亚梅, 张国军, 陈燕, 王谦, 马瑞敏, 吕虹, 康熙雄

首都医科大学附属北京天坛医院检验科, 北京 100050

收稿日期:2014-06-18 出版日期:2014-08-21 发布日期:2014-07-22
通讯作者: 张国军 E-mail:tiantanzgj@163.com
基金资助:
国家科技支撑计划课题（2013BAI17B04）;北京市卫生系统高层次卫生技术人才培养计划（2013-3-052）。

Effect of sample storage method and time on the test results for serum total carbon dioxide

Du Yamei, Zhang Guojun, Chen Yan, Wang Qian, Ma Ruimin, Lyu Hong, Kang Xixiong

Clinical Laboratory Diagnosis Center, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China

Received:2014-06-18 Online:2014-08-21 Published:2014-07-22
Supported by:
This study was supported by State Science and Technology Support Program(2013BAI17B04)and High Level Technical Talent Development of Beijing Health System(2013-3-052).

摘要/Abstract

摘要：

目的研究不同的标本保存方法和时间对血清总二氧化碳（total carbon dioxide，TCO₂）检测结果的影响。方法保证TCO₂检测系统稳定性符合实验室质量目标的前提下，利用高值QC（quality control）、低值QC、混合血清3种样本，分别检测室温敞口、室温封口、4℃敞口、4℃封口时不同时间点TCO₂的浓度，计算TCO₂浓度随时间的变化率。结果高值QC、低值QC、混合血清在室温敞口保存75 min时，TCO₂结果分别下降了9.65%、13.42 %、7.74%；4℃敞口保存75 min时，TCO₂结果分别下降了8.96%、8.60%、3.94%；在室温封口保存90 min时，TCO₂结果分别下降了4.53%、9.43%、3.94%；在4℃封口保存2 h，TCO₂结果仅分别下降了3.44%、3.56 %、2.19%。结论随着标本保存时间的延长血清TCO₂浓度不断下降，并且不同的保存方法下降的速率不同。封口保存TCO₂结果下降低于敞口状态，保存温度越低TCO₂结果下降越慢。

关键词: 血清总二氧化碳, 自动生化分析仪, 实验室质量控制

Abstract:

Objective To study the effect of sample storage method and time on the test results of serum total carbon dioxide. Methods Under the condition that total carbon dioxide(TCO₂) measurement system can guarantee the stability of TCO₂ results. TCO₂ of two quality controls and a mixed serum was measured promptly at different time points after storage under various conditions: open samples at room temperature(25±2)℃, sealed samples at room temperature, open samples at 4℃, and sealed samples at 4℃. Results High level quality control(QC), low level QC and mixed serum samples decreased by 9.65%, 13.42% and 7.74%, respectively, at 75 min when samples were stored at room temperature and kept open. The same samples decreased by 4.53, 9.43 and 3.94% at 90 min when stored at room temperature and kept seal. The samples decreased by 8.96, 8.60 and 3.94% at 75 min when stored at 4℃ and kept open, and decreased by 3.44%, 3.56% and 2.19% at 2 h when stored at 4℃ and kept sealed. Conclusion TCO₂ decreased over time and decreased at different rate according to storage condition. TCO₂ of sealed samples decreased much slower than open samples. The colder samples stored, the slower TCO₂ decreased.

Key words: serum total CO₂, automatic biochemistry analyzer, laboratory quality management

中图分类号:

R446.11

杜亚梅, 张国军, 陈燕, 王谦, 马瑞敏, 吕虹, 康熙雄. 标本保存方法和时间对血清总二氧化碳结果的影响[J]. 首都医科大学学报, 2014, 35(4): 438-442.

Du Yamei, Zhang Guojun, Chen Yan, Wang Qian, Ma Ruimin, Lyu Hong, Kang Xixiong. Effect of sample storage method and time on the test results for serum total carbon dioxide[J]. Journal of Capital Medical University, 2014, 35(4): 438-442.

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标本保存方法和时间对血清总二氧化碳结果的影响

Effect of sample storage method and time on the test results for serum total carbon dioxide

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