离子通道和机械负荷对耳蜗外毛细胞电致运动的影响

doi:10.3969/j.issn.1006-7795.2014.04.019

首都医科大学学报 ›› 2014, Vol. 35 ›› Issue (4): 477-482.doi: 10.3969/j.issn.1006-7795.2014.04.019

离子通道和机械负荷对耳蜗外毛细胞电致运动的影响

李轶¹, 贾淑萍², 龚树生¹, 何志洲²

1. 首都医科大学附属北京同仁医院耳鼻咽喉头颈外科, 北京 100730;
2. Department of Biomedical Sciences, Creighton University, Omaha 68178, USA

收稿日期:2014-07-04 出版日期:2014-08-21 发布日期:2014-07-22
通讯作者: 龚树生 E-mail:gongss@ccmu.edu.cn
基金资助:
国家重点基础研究发展计划（973计划）（2011CB504503）资助项目。

Mechanical responses of cochlear outer hair cells

Li Yi¹, Liu Huizhan², Gong Shusheng¹, He Zhizhou²

1. Department of Otorhinolaryngology-Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China;
2. Department of Biomedical Sciences, Creighton University, Omaha 68178, USA

Received:2014-07-04 Online:2014-08-21 Published:2014-07-22
Supported by:
This study was supported by National Basic Research Program of China(2011CB504503).

摘要/Abstract

摘要：

目的研究豚鼠耳蜗外毛细胞的细胞膜电导和机械负荷对细胞电致运动的动力学特性的影响。方法使用分离自豚鼠耳蜗第四回的外毛细胞，采用全细胞电压钳和微室电极技术。使用以光电二极管为基础的测量系统来测量电致运动。结果外毛细胞的膜电导可以同时影响电致运动的灵敏度和运动的非线性，而机械负载可以显著影响电致运动的幅度、不对称和频率响应。结论外毛细胞的膜电导和机械负荷可以影响外毛细胞的电致运动，从而影响耳蜗放大器功能。

关键词: 外毛细胞, 电致运动, 机械负荷, 频率响应, 豚鼠

Abstract:

Objective To examine the influence of membrane conductances and mechanical load on the dynamic characteristics of outer hair cell(OHC) motility. Methods All the experiments were performed on OHCs isolated from the 4th turns of guinea pig cochleae, using whole-cell voltage-clamp and microchamber techniques. Motility was measured using a photodiode-based measurement system. Results Membrane conductances influence both sensitivity and nonlinearity of motility, and that mechanical load significantly affects magnitude, asymmetry and frequency response of motility. Conclusion Membrane conductances and mechanical load influence OHC motility, thereby, the cochlear amplifier.

Key words: outer hair cell, motility, mechanical load, frequency response, guinea pig

中图分类号:

R764.35

李轶, 贾淑萍, 龚树生, 何志洲. 离子通道和机械负荷对耳蜗外毛细胞电致运动的影响[J]. 首都医科大学学报, 2014, 35(4): 477-482.

Li Yi, Liu Huizhan, Gong Shusheng, He Zhizhou. Mechanical responses of cochlear outer hair cells[J]. Journal of Capital Medical University, 2014, 35(4): 477-482.

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离子通道和机械负荷对耳蜗外毛细胞电致运动的影响

Mechanical responses of cochlear outer hair cells

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