高白细胞急性髓系白血病TGF-β1的表达及其临床意义

doi:10.3969/j.issn.1006-7795.2014.05.006

首都医科大学学报 ›› 2014, Vol. 35 ›› Issue (5): 555-559.doi: 10.3969/j.issn.1006-7795.2014.05.006

高白细胞急性髓系白血病TGF-β₁的表达及其临床意义

王敏¹, 王立茹¹, 崔建英¹, 周合冰²

1. 首都医科大学附属复兴医院血液内科, 北京 100038;
2. 首都医科大学潞河教学医院血液内科, 北京 101149

收稿日期:2014-08-25 出版日期:2014-10-21 发布日期:2014-10-20
通讯作者: 王立茹 E-mail:wanglirumail@126.com
基金资助:
2007卫生部公益性行业科研专项（200802031）。

Expression of TGF-β₁ in hyperleukocytic acute myeloid leukemia and its clinical significance

Wang Min¹, Wang Liru¹, Cui Jianying¹, Zhou Hebing²

1. Department of Hematology, Fuxing Hospital, Capital Medical University, Beijing 100038, China;
2. Department of Hematology, Luhe Hospital, Capital Medical University, Beijing 101149, China

Received:2014-08-25 Online:2014-10-21 Published:2014-10-20
Supported by:
This study was supported by 2007 Department of Health Public Industry Scientific Research(200802031).

摘要/Abstract

摘要：

目的本文旨在探讨转化生长因子β-1（transform growth factor β₁， TGF-β₁）在高白细胞急性髓系白血病（acute myeloid leukemia， AML）发病中的作用。方法分别采用实时定量PCR、流式细胞术及酶联免疫反应检测38例AML患者骨髓单个核细胞（bone marrow mononuclear cells， BMMNC）内TGF-β₁ mRNA浓度、48例AML患者BMMNC的TGF-β₁蛋白和40例AML患者血清TGF-β₁蛋白质量浓度。结果高白细胞AML患者、非高白细胞AML患者和正常对照者的TGF-β₁ mRNA浓度差异无统计学意义，高白细胞和非高白细胞AML患者的胞内TGF-β₁蛋白质量浓度显著高于正常对照者，血清TGF-β₁蛋白质量浓度显著低于正常对照者。高白细胞和非高白细胞AML患者之间的胞内和血清TGF-β₁蛋白质量浓度差异无统计学意义。高白细胞与非高白细胞AML患者之间胞内及血清TGF-β₁质量浓度与FAB亚型无关。结论 AML患者血清TGF-β₁浓度降低可能参与了疾病的发生，而血清TGF-β₁的减少可能并不能反映BMMNC合成TGF-β₁减少。TGF-β₁对高白细胞AML的发病并未产生重要影响。

关键词: 转化生长因子β-1, 急性髓系白血病, 高白细胞

Abstract:

Objective To investigate the role of transform growth factor β₁ (TGF-β₁) in pathogenesis of hyperleukocytic acute myeloid leukemia (AML).Methods The mRNA transcription levels of TGF-β₁ from bone marrow mononuclear cells (BMMNC) in 38 AML patients, protein expression levels of intracellular TGF-β₁ from BMMNC in 48 AML patients, and serum levels of TGF-β₁ from 40 AML patients were measured by real-time fluorescence quantitative polymerase chain reaction (RQ-PCR), flow cytometry, and double-antibody sandwich enzyme-linked immunosorbent assay (ELISA), respectively. Results The relative levels of the TGF-β₁ gene expression among hyperleukocytic AML patients, non-hyperleukocytic AML patients, and NC has no significant difference (P>0.05). The protein expression levels of TGF-β₁ in both hyperleukocytic AML and non-hyperleukocytic AML patients were significantly higher than that in NC (P<0.05), the plasma levels were significantly lower than that in NC (P<0.05). There was no significant difference in intracellular protein expression levels and plasma levels of TGF-β₁ between hyperleukocytic AML and non-hyperleukocytic AML patients (P>0.05). There was no significant difference in intracellular protein expression levels and plasma levels of TGF-β₁ between the FAB subtypes in hyperleukocytic and non-hyperleukocytic AML (P>0.05). Conclusion Decreased serum levels of TGF-β₁ may be involved in the pathogenesis of AML. However, decreased TGF-β₁ serum levels may not reflect reduced TGF-β₁ production by BMMNC. Our study showed that TGF-β₁ had no important impact on the pathogenesis of hyperleukocytic AML.

Key words: transform growth factor β₁, acute myeloid leukemia, hyperleukocytic

中图分类号:

R733.71

王敏, 王立茹, 崔建英, 周合冰. 高白细胞急性髓系白血病TGF-β₁的表达及其临床意义[J]. 首都医科大学学报, 2014, 35(5): 555-559.

Wang Min, Wang Liru, Cui Jianying, Zhou Hebing. Expression of TGF-β₁ in hyperleukocytic acute myeloid leukemia and its clinical significance[J]. Journal of Capital Medical University, 2014, 35(5): 555-559.

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高白细胞急性髓系白血病TGF-β₁的表达及其临床意义

Expression of TGF-β₁ in hyperleukocytic acute myeloid leukemia and its clinical significance

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