siRNA干扰TRIM29基因对人肺癌NCI-H520细胞株增生及迁移能力的影响

doi:10.3969/j.issn.1006-7795.2014.06.008

首都医科大学学报 ›› 2014, Vol. 35 ›› Issue (6): 717-720.doi: 10.3969/j.issn.1006-7795.2014.06.008

• 胸外科学:从基础到临床 • 上一篇下一篇

siRNA干扰TRIM29基因对人肺癌NCI-H520细胞株增生及迁移能力的影响

刘春晓, 李辉, 侯生才, 胡滨, 苗劲柏, 张文谦

首都医科大学附属北京朝阳医院胸外科, 北京 100020

收稿日期:2014-10-16 发布日期:2014-12-15
通讯作者: 李辉 E-mail:huilee@vip.sina.com
基金资助:
高等学校博士学科点专项科研基金(20111107110003)

Inhibition of TRIM29 gene by siRNA suppresses the proliferation and migration of NCI-H520 lung cancer cells

Liu Chunxiao, LI Hui, Hou Shengcai, Hu Bin, Miao Jinbai, Zhang Wenqian

Department of Thoracic Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020

Received:2014-10-16 Published:2014-12-15
Supported by:
This study was supported by Specialized Research Fund for the Doctoral Program of Higher Education(20111107110003).

摘要/Abstract

摘要：

目的探讨TRIM29基因沉默对肺癌细胞株NCI-H520增生和迁移能力的影响。方法将针对TRIM29的siRNA导入NCI-H520细胞,用real time PCR和Western blotting法分析TRIM29基因及蛋白表达情况,MTT法和Transwell小室法检测其增生、迁移能力。结果 NCI-H520细胞转染48 h后,与空白组及对照组相比,TRIM29 siRNA转染组TRIM29 mRNA和蛋白表达均明显下调,细胞生长明显减慢,细胞迁移能力下降。结论 siRNA干扰下调TRIM29基因表达能抑制肺癌细胞NCI-H520的增生和迁移能力。

关键词: 肺癌, RNA干扰技术, TRIM29蛋白, 细胞增生, 细胞迁移

Abstract:

Objective To study the effects of TRIM29 gene on the proliferation and migration of human lung cancer cell line NCI-H520. Methods We transfected TRIM29 siRNA into NCI-H520 cells. Real time reverse transcriptase polymerase chain reaction and Western blotting assay were employed to determine TRIM29 messenger (m)RNA and protein expressions. MTT assay was used to investigate the proliferation of the cells, and the cell migration was evaluated using a transwell migration assay. Results The mRNA and protein levels of TRIM29 were significantly downregulated at 48 h after transfection in TRIM29 siRNA group compared with the control groups. The cell proliferation and migration were significantly reduced. Conclusion siRNA targeting TRIM29 gene can inhibit the proliferation and migration of human lung cancer cell line NCI-H520.

Key words: lung cancer, RNA interference, TRIM29 protein, cell proliferation, cell migration

中图分类号:

R734.2

刘春晓, 李辉, 侯生才, 胡滨, 苗劲柏, 张文谦. siRNA干扰TRIM29基因对人肺癌NCI-H520细胞株增生及迁移能力的影响[J]. 首都医科大学学报, 2014, 35(6): 717-720.

Liu Chunxiao, LI Hui, Hou Shengcai, Hu Bin, Miao Jinbai, Zhang Wenqian. Inhibition of TRIM29 gene by siRNA suppresses the proliferation and migration of NCI-H520 lung cancer cells[J]. Journal of Capital Medical University, 2014, 35(6): 717-720.

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siRNA干扰TRIM29基因对人肺癌NCI-H520细胞株增生及迁移能力的影响

Inhibition of TRIM29 gene by siRNA suppresses the proliferation and migration of NCI-H520 lung cancer cells

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