寡聚化和磷酸化α-突触核蛋白在不同年龄段食蟹猴脑不同部位突触小体中的表达分布

doi:10.3969/j.issn.1006-7795.2015.05.001

首都医科大学学报 ›› 2015, Vol. 36 ›› Issue (5): 671-674.doi: 10.3969/j.issn.1006-7795.2015.05.001

• PD的发病机制与治疗 • 上一篇下一篇

寡聚化和磷酸化α-突触核蛋白在不同年龄段食蟹猴脑不同部位突触小体中的表达分布

杨巍巍^1,2, 李昕^1,2, 李旭冉^1,2, 李旭颖^1,2, 于顺^1,2,3

1. 首都医科大学宣武医院神经生物学研究室, 北京 100053;
2. 帕金森病研究北京重点实验室, 北京 100053;
3. 北京脑重大疾病研究院帕金森病研究所, 北京 100053

收稿日期:2015-09-14 出版日期:2015-10-21 发布日期:2015-10-20
基金资助:
国家重点基础研究发展计划(2011CB504101),国家自然科学基金(81071014, 81371200, 81401042),国家科技支撑计划课题(2012BAI10B03),北京市自然科学基金(7122035),首都卫生发展科研专项课题(2011-4001-01)。

Differential expressions of oligomer α-synuclein and phosphorylated α-synuclein in synaptosome in the brain regions of different ages of cynomolgus monkeys of different ages

Yang Weiwei^1,2, Li Xin^1,2, Li Xuran^1,2, Li Xuying^1,2, Yu Shun^1,2,3

1. Department of Neurobiology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China;
2. Key Laboratory of Neurodegenerative Disease, Ministry of Education, Beijing 100053, China;
3. Center for Parkinson's Disease, Beijing Institute for Brain Disorders, Beijing 100053, China

Received:2015-09-14 Online:2015-10-21 Published:2015-10-20
Contact: 于顺 E-mail:yushun103@163.com
Supported by:
This study was supported by Major State Basic Research Development Program of China (973 Program)(2011CB504101), Natural Science Foundation of China (81071014, 81371200, 81401042), National Science and Technology Support Program (2012BAI10B03), Natural Science Foundation of Beijing (7122035), The Capital Health Research and Development Special Fund (2011-4001-01).

摘要/Abstract

摘要： 目的研究寡聚化(oligomer-α-synuclein,oligo-α-Syn)和磷酸化α-突触核蛋白(phosphorylated-α-Synuclein,p-α-Syn)在不同年龄段食蟹猴脑不同部位突触小体中的表达差异。方法酶联免疫吸附实验(enzyme linked immunosorbent assay, ELISA)检测oligo-α-Syn与p-α-Syn在不同年龄段食蟹猴脑不同部位突触小体中的表达。结果在老年食蟹猴的前额叶、枕叶、颞叶、海马、纹状体和丘脑中,突触小体中寡聚化与磷酸化α-Syn表达较青年与中年猴显著增加,且差异有统计学意义(P<0.01)。结论食蟹猴颞叶、海马、纹状体和丘脑突触小体中α-Syn寡聚化与磷酸化表达呈增龄性增加。

关键词: 寡聚化α-突触核蛋白, 磷酸化α-突触核蛋白, 食蟹猴, 突触小体

Abstract: Objective To explore the expressions of oligomer α-synuclein (oligo-α-syn) and phosphorylated α-synuclein (p-α-syn) in synaptosome in brain regions of cynomolgus monkeys of different ages. Methods ELISA was applied to measure the expressions of oligo-α-syn and p-α-syn in different brain regions of cynomolgus monkeys of different ages. Results The synaptosome-α-Syn oligomers and synaptosome-p-α-syn increased significantly in prefrontal lobe, occipital lobe, temporal lobe, hippocampus, striatum and thalamus of old monkeys compared with that of young and middle-aged monkeys (P<0.01). Conclusion The highest level expression of oligo-α-syn and p-α-syn was found in synaptosome of all brain regions in old cynomolgus monkeys.

Key words: oligomer α-synuclein (oligo-α-syn), phosphorylation-α-synuclein (p-α-Syn), cynomolgus monkeys, synaptosome

中图分类号:

R338

杨巍巍, 李昕, 李旭冉, 李旭颖, 于顺. 寡聚化和磷酸化α-突触核蛋白在不同年龄段食蟹猴脑不同部位突触小体中的表达分布[J]. 首都医科大学学报, 2015, 36(5): 671-674.

Yang Weiwei, Li Xin, Li Xuran, Li Xuying, Yu Shun. Differential expressions of oligomer α-synuclein and phosphorylated α-synuclein in synaptosome in the brain regions of different ages of cynomolgus monkeys of different ages[J]. Journal of Capital Medical University, 2015, 36(5): 671-674.

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寡聚化和磷酸化α-突触核蛋白在不同年龄段食蟹猴脑不同部位突触小体中的表达分布

Differential expressions of oligomer α-synuclein and phosphorylated α-synuclein in synaptosome in the brain regions of different ages of cynomolgus monkeys of different ages

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